TUTCRIS - Tampereen teknillinen yliopisto


Engineering and characterization of bacterial nanocellulose as a future biomaterial for wide range of applications

Tutkimustuotos: Konferenssiesitys, posteri tai abstrakti


TilaJulkaistu - 18 lokakuuta 2017


Nature’s biomaterial, cellulose, traditionally obtained from plant sources, has been used in various industrial applications such as fabrics. However, the cellulose contents is extracted by the expense of strong physical-chemical, high energy input pretreatment methods resulting in increased costs and generation of toxic sulfoxide residues and/or lignin derived by-products. Thus, plant cellulose is not suitable for biomedical applications like artificial skin to treat burn wounds. Certain bacteria, living in rotten fruits, are capable of naturally producing pure and highly crystalline cellulose, providing an alternative sustainable means of production. Even waste materials can be used as substrates. The most efficient cellulose producers are found in the genus Komagataeibacter, of which the strain Komagataeibacter xylinus represents the most often used organism for studying cellulose biogenesis. The highly ordered bacterial cellulose (BC) is synthesized by bacterial cellulose synthase (bcs) operon consisting of four genes bcsA, bcsB, bcsC and bcsD.Here, the multienzyme complex genes (bcs operon) in K. xylinus were over expressed through genetic engineering either separately or all together (see Figure) resulting in complete utilization of glucose substrate in four days cultivation in static conditions. The bacterial nanocellulose films produced by the engineered bacterium were characterized (mechanical and structural) and analyzed for their piezoelectric sensitivities. [1] Keywords: Bacterial nanocellulose, biomaterial, genetic engineering, Komagataeibacter xylinus (1] Rahul Mangayil, Satu Rajala, Arno Pammo, Essi Sarlin, Jin Luo, Ville Santala, Matti Karp and Sampo Tuukkanen (2017) Engineering and Characterization of Bacterial Nanocellulose Films as Low Cost and Flexible Sensor Material ACS Applied Materials & Interfaces, doi: 10.1021/acsami.7b04927.