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Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools

Tutkimustuotosvertaisarvioitu

Standard

Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools. / Palmroth, Aleksi; Pitkänen, Sanna; Hannula, Markus; Paakinaho, Kaarlo; Hyttinen, Jari; Miettinen, Susanna; Kellomäki, Minna.

julkaisussa: Journal of the Royal Society. Interface, Vuosikerta 17, Nro 165, 20200102, 01.04.2020.

Tutkimustuotosvertaisarvioitu

Harvard

Palmroth, A, Pitkänen, S, Hannula, M, Paakinaho, K, Hyttinen, J, Miettinen, S & Kellomäki, M 2020, 'Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools', Journal of the Royal Society. Interface, Vuosikerta. 17, Nro 165, 20200102. https://doi.org/10.1098/rsif.2020.0102

APA

Palmroth, A., Pitkänen, S., Hannula, M., Paakinaho, K., Hyttinen, J., Miettinen, S., & Kellomäki, M. (2020). Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools. Journal of the Royal Society. Interface, 17(165), [20200102]. https://doi.org/10.1098/rsif.2020.0102

Vancouver

Palmroth A, Pitkänen S, Hannula M, Paakinaho K, Hyttinen J, Miettinen S et al. Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools. Journal of the Royal Society. Interface. 2020 huhti 1;17(165). 20200102. https://doi.org/10.1098/rsif.2020.0102

Author

Palmroth, Aleksi ; Pitkänen, Sanna ; Hannula, Markus ; Paakinaho, Kaarlo ; Hyttinen, Jari ; Miettinen, Susanna ; Kellomäki, Minna. / Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools. Julkaisussa: Journal of the Royal Society. Interface. 2020 ; Vuosikerta 17, Nro 165.

Bibtex - Lataa

@article{5d40eb52f1a94394a45a5c2e5c94b9db,
title = "Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools",
abstract = "Micro-computed tomography (micro-CT) provides a means to analyse and model three-dimensional (3D) tissue engineering scaffolds. This study proposes a set of micro-CT-based tools firstly for evaluating the microstructure of scaffolds and secondly for comparing different cell seeding methods. The pore size, porosity and pore interconnectivity of supercritical CO2 processed poly(l-lactide-co-ɛ-caprolactone) (PLCL) and PLCL/β-tricalcium phosphate scaffolds were analysed using computational micro-CT models. The models were supplemented with an experimental method, where iron-labelled microspheres were seeded into the scaffolds and micro-CT imaged to assess their infiltration into the scaffolds. After examining the scaffold architecture, human adipose-derived stem cells (hASCs) were seeded into the scaffolds using five different cell seeding methods. Cell viability, number and 3D distribution were evaluated. The distribution of the cells was analysed using micro-CT by labelling the hASCs with ultrasmall paramagnetic iron oxide nanoparticles. Among the tested seeding methods, a forced fluid flow-based technique resulted in an enhanced cell infiltration throughout the scaffolds compared with static seeding. The current study provides an excellent set of tools for the development of scaffolds and for the design of 3D cell culture experiments.",
keywords = "cell seeding, micro-CT, microsphere, supercritical CO2 processing, tissue engineering, USPIO",
author = "Aleksi Palmroth and Sanna Pitk{\"a}nen and Markus Hannula and Kaarlo Paakinaho and Jari Hyttinen and Susanna Miettinen and Minna Kellom{\"a}ki",
note = "INT=bmte,{"}Miettinen, Susanna{"}",
year = "2020",
month = "4",
day = "1",
doi = "10.1098/rsif.2020.0102",
language = "English",
volume = "17",
journal = "Journal of the Royal Society. Interface",
issn = "1742-5689",
publisher = "Royal Society, The",
number = "165",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools

AU - Palmroth, Aleksi

AU - Pitkänen, Sanna

AU - Hannula, Markus

AU - Paakinaho, Kaarlo

AU - Hyttinen, Jari

AU - Miettinen, Susanna

AU - Kellomäki, Minna

N1 - INT=bmte,"Miettinen, Susanna"

PY - 2020/4/1

Y1 - 2020/4/1

N2 - Micro-computed tomography (micro-CT) provides a means to analyse and model three-dimensional (3D) tissue engineering scaffolds. This study proposes a set of micro-CT-based tools firstly for evaluating the microstructure of scaffolds and secondly for comparing different cell seeding methods. The pore size, porosity and pore interconnectivity of supercritical CO2 processed poly(l-lactide-co-ɛ-caprolactone) (PLCL) and PLCL/β-tricalcium phosphate scaffolds were analysed using computational micro-CT models. The models were supplemented with an experimental method, where iron-labelled microspheres were seeded into the scaffolds and micro-CT imaged to assess their infiltration into the scaffolds. After examining the scaffold architecture, human adipose-derived stem cells (hASCs) were seeded into the scaffolds using five different cell seeding methods. Cell viability, number and 3D distribution were evaluated. The distribution of the cells was analysed using micro-CT by labelling the hASCs with ultrasmall paramagnetic iron oxide nanoparticles. Among the tested seeding methods, a forced fluid flow-based technique resulted in an enhanced cell infiltration throughout the scaffolds compared with static seeding. The current study provides an excellent set of tools for the development of scaffolds and for the design of 3D cell culture experiments.

AB - Micro-computed tomography (micro-CT) provides a means to analyse and model three-dimensional (3D) tissue engineering scaffolds. This study proposes a set of micro-CT-based tools firstly for evaluating the microstructure of scaffolds and secondly for comparing different cell seeding methods. The pore size, porosity and pore interconnectivity of supercritical CO2 processed poly(l-lactide-co-ɛ-caprolactone) (PLCL) and PLCL/β-tricalcium phosphate scaffolds were analysed using computational micro-CT models. The models were supplemented with an experimental method, where iron-labelled microspheres were seeded into the scaffolds and micro-CT imaged to assess their infiltration into the scaffolds. After examining the scaffold architecture, human adipose-derived stem cells (hASCs) were seeded into the scaffolds using five different cell seeding methods. Cell viability, number and 3D distribution were evaluated. The distribution of the cells was analysed using micro-CT by labelling the hASCs with ultrasmall paramagnetic iron oxide nanoparticles. Among the tested seeding methods, a forced fluid flow-based technique resulted in an enhanced cell infiltration throughout the scaffolds compared with static seeding. The current study provides an excellent set of tools for the development of scaffolds and for the design of 3D cell culture experiments.

KW - cell seeding

KW - micro-CT

KW - microsphere

KW - supercritical CO2 processing

KW - tissue engineering

KW - USPIO

U2 - 10.1098/rsif.2020.0102

DO - 10.1098/rsif.2020.0102

M3 - Article

VL - 17

JO - Journal of the Royal Society. Interface

JF - Journal of the Royal Society. Interface

SN - 1742-5689

IS - 165

M1 - 20200102

ER -