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Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating

Tutkimustuotosvertaisarvioitu

Standard

Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating. / Guelfi, Gabriella; Stefanetti, Valentina; Zampini, Danilo; Oommen, Oommen P.; Brecchia, Gabriele; Dall'Aglio, Cecilia; Arcelli, Rolando; Cochetti, Giovanni; Boni, Andrea; Mearini, Ettore.

julkaisussa: Scientific Reports, Vuosikerta 7, Nro 1, 10355, 01.12.2017.

Tutkimustuotosvertaisarvioitu

Harvard

Guelfi, G, Stefanetti, V, Zampini, D, Oommen, OP, Brecchia, G, Dall'Aglio, C, Arcelli, R, Cochetti, G, Boni, A & Mearini, E 2017, 'Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating', Scientific Reports, Vuosikerta. 7, Nro 1, 10355. https://doi.org/10.1038/s41598-017-09872-0

APA

Guelfi, G., Stefanetti, V., Zampini, D., Oommen, O. P., Brecchia, G., Dall'Aglio, C., ... Mearini, E. (2017). Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating. Scientific Reports, 7(1), [10355]. https://doi.org/10.1038/s41598-017-09872-0

Vancouver

Guelfi G, Stefanetti V, Zampini D, Oommen OP, Brecchia G, Dall'Aglio C et al. Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating. Scientific Reports. 2017 joulu 1;7(1). 10355. https://doi.org/10.1038/s41598-017-09872-0

Author

Guelfi, Gabriella ; Stefanetti, Valentina ; Zampini, Danilo ; Oommen, Oommen P. ; Brecchia, Gabriele ; Dall'Aglio, Cecilia ; Arcelli, Rolando ; Cochetti, Giovanni ; Boni, Andrea ; Mearini, Ettore. / Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating. Julkaisussa: Scientific Reports. 2017 ; Vuosikerta 7, Nro 1.

Bibtex - Lataa

@article{74af4df805c949efb495463ee121058c,
title = "Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating",
abstract = "This study investigated the location of hyaluronic acid (HA)-A nd chondroitin sulphate (CS)-coated gold nanoparticles in rabbit bladder and evaluated gene expression of CD44, RHAMM and ICAM-1 receptors involved in HA and CS transport into the cell. Gold nanoparticles were synthesised by reduction of gold salts with HA or CS to form HA-AuNPs and CS-AuNPs. Bladder samples were incubated with CS-AuNPs and HA-AuNPs or without glycosaminoglycans. Transmission electron microscopy, optic microscopy and scanning electron microscopy were used to determine the location of the synthesised AuNPs. Real-time PCR was used to analyse expression of urothelial cell receptors CD44, RHAMM, ICAM-1, after ex vivo administration of CS-AuNPs and HA-AuNPs. We showed that HA-AuNPs and CS-AuNPs were located in the cytoplasm and tight junctions of urothelial umbrella cells; this appearance was absent in untreated bladders. There were no significant differences in gene expression levels for CD44, RHAMM and ICAM-1 receptors in treated versus control bladder tissues. In conclusion, we clearly showed the presence of exogenous GAGs in the bladder surface and the tight junctions between umbrella cells, which is important in the regeneration pathway of the urothelium. The GAGs-AuNPs offer a promising approach to understanding the biophysical properties and imaging of urothelial tissue.",
author = "Gabriella Guelfi and Valentina Stefanetti and Danilo Zampini and Oommen, {Oommen P.} and Gabriele Brecchia and Cecilia Dall'Aglio and Rolando Arcelli and Giovanni Cochetti and Andrea Boni and Ettore Mearini",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-09872-0",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating

AU - Guelfi, Gabriella

AU - Stefanetti, Valentina

AU - Zampini, Danilo

AU - Oommen, Oommen P.

AU - Brecchia, Gabriele

AU - Dall'Aglio, Cecilia

AU - Arcelli, Rolando

AU - Cochetti, Giovanni

AU - Boni, Andrea

AU - Mearini, Ettore

PY - 2017/12/1

Y1 - 2017/12/1

N2 - This study investigated the location of hyaluronic acid (HA)-A nd chondroitin sulphate (CS)-coated gold nanoparticles in rabbit bladder and evaluated gene expression of CD44, RHAMM and ICAM-1 receptors involved in HA and CS transport into the cell. Gold nanoparticles were synthesised by reduction of gold salts with HA or CS to form HA-AuNPs and CS-AuNPs. Bladder samples were incubated with CS-AuNPs and HA-AuNPs or without glycosaminoglycans. Transmission electron microscopy, optic microscopy and scanning electron microscopy were used to determine the location of the synthesised AuNPs. Real-time PCR was used to analyse expression of urothelial cell receptors CD44, RHAMM, ICAM-1, after ex vivo administration of CS-AuNPs and HA-AuNPs. We showed that HA-AuNPs and CS-AuNPs were located in the cytoplasm and tight junctions of urothelial umbrella cells; this appearance was absent in untreated bladders. There were no significant differences in gene expression levels for CD44, RHAMM and ICAM-1 receptors in treated versus control bladder tissues. In conclusion, we clearly showed the presence of exogenous GAGs in the bladder surface and the tight junctions between umbrella cells, which is important in the regeneration pathway of the urothelium. The GAGs-AuNPs offer a promising approach to understanding the biophysical properties and imaging of urothelial tissue.

AB - This study investigated the location of hyaluronic acid (HA)-A nd chondroitin sulphate (CS)-coated gold nanoparticles in rabbit bladder and evaluated gene expression of CD44, RHAMM and ICAM-1 receptors involved in HA and CS transport into the cell. Gold nanoparticles were synthesised by reduction of gold salts with HA or CS to form HA-AuNPs and CS-AuNPs. Bladder samples were incubated with CS-AuNPs and HA-AuNPs or without glycosaminoglycans. Transmission electron microscopy, optic microscopy and scanning electron microscopy were used to determine the location of the synthesised AuNPs. Real-time PCR was used to analyse expression of urothelial cell receptors CD44, RHAMM, ICAM-1, after ex vivo administration of CS-AuNPs and HA-AuNPs. We showed that HA-AuNPs and CS-AuNPs were located in the cytoplasm and tight junctions of urothelial umbrella cells; this appearance was absent in untreated bladders. There were no significant differences in gene expression levels for CD44, RHAMM and ICAM-1 receptors in treated versus control bladder tissues. In conclusion, we clearly showed the presence of exogenous GAGs in the bladder surface and the tight junctions between umbrella cells, which is important in the regeneration pathway of the urothelium. The GAGs-AuNPs offer a promising approach to understanding the biophysical properties and imaging of urothelial tissue.

U2 - 10.1038/s41598-017-09872-0

DO - 10.1038/s41598-017-09872-0

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 10355

ER -