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HPLC-SEC: a new approach to characterise complex wastewater effluents

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Standard

HPLC-SEC : a new approach to characterise complex wastewater effluents. / Szabo, Hilda Marta; Lepistö, Raghida; Tuhkanen, Tuula.

julkaisussa: International Journal of Environmental Analytical Chemistry, Vuosikerta 96, Nro 3, 19.02.2016, s. 257-270.

Tutkimustuotosvertaisarvioitu

Harvard

Szabo, HM, Lepistö, R & Tuhkanen, T 2016, 'HPLC-SEC: a new approach to characterise complex wastewater effluents', International Journal of Environmental Analytical Chemistry, Vuosikerta. 96, Nro 3, Sivut 257-270. https://doi.org/10.1080/03067319.2016.1150463

APA

Szabo, H. M., Lepistö, R., & Tuhkanen, T. (2016). HPLC-SEC: a new approach to characterise complex wastewater effluents. International Journal of Environmental Analytical Chemistry, 96(3), 257-270. https://doi.org/10.1080/03067319.2016.1150463

Vancouver

Szabo HM, Lepistö R, Tuhkanen T. HPLC-SEC: a new approach to characterise complex wastewater effluents. International Journal of Environmental Analytical Chemistry. 2016 helmi 19;96(3):257-270. https://doi.org/10.1080/03067319.2016.1150463

Author

Szabo, Hilda Marta ; Lepistö, Raghida ; Tuhkanen, Tuula. / HPLC-SEC : a new approach to characterise complex wastewater effluents. Julkaisussa: International Journal of Environmental Analytical Chemistry. 2016 ; Vuosikerta 96, Nro 3. Sivut 257-270.

Bibtex - Lataa

@article{2cedf467223a4f37b98d6e762ce5f360,
title = "HPLC-SEC: a new approach to characterise complex wastewater effluents",
abstract = "This work investigates the use of HPLC-SEC to characterise dissolved organic matter (DOM) of complex wastewater effluents. A silica-based column, sodium acetate eluent and multiple detections were employed: UV-254 absorbance for humictype, and tryptophan-like (Ex/Em = 270/355) and tyrosine-like (Ex/Em = 270/310) fluorescence for protein type compounds. Effects of eluent pH, eluent ionic strength and injection volume on separation efficiency were tested. Humic-type and protein-type fractions were clearly differentiated and eluted within and out of calibration range. Eluent ionic strength had the greatest influence on global resolution; the lowest eluent concentration of 0.01 M produced the best separation for all wastewater effluents tested at any detection. UV-254 absorbance was higher at neutral and basic eluent pH while tryptophan-like fluorescence depended on the sample composition rather than on the eluent pH or ionic strength. Tyrosine-like fluorescence decreased significantly with the increase of eluent ionic strength. Accurate molecular weight measurements could not be done, the separation being influenced by secondary interactions, but could be approximated using separate calibrations with sodium salts of polystyrene-sulfonates and protein standards. The results show that this method is suitable for determining DOM in wastewater at low eluent concentrations (up to 0.03 M), at neutral or slightly basic pH.",
keywords = "DOM, Fluorescence, greywater, humic, ionic strength, proteins, tryptophan, UV-254",
author = "Szabo, {Hilda Marta} and Raghida Lepist{\"o} and Tuula Tuhkanen",
note = "EXT={"}Tuhkanen, Tuula{"}",
year = "2016",
month = "2",
day = "19",
doi = "10.1080/03067319.2016.1150463",
language = "English",
volume = "96",
pages = "257--270",
journal = "International Journal of Environmental Analytical Chemistry",
issn = "0306-7319",
publisher = "Taylor & Francis",
number = "3",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - HPLC-SEC

T2 - a new approach to characterise complex wastewater effluents

AU - Szabo, Hilda Marta

AU - Lepistö, Raghida

AU - Tuhkanen, Tuula

N1 - EXT="Tuhkanen, Tuula"

PY - 2016/2/19

Y1 - 2016/2/19

N2 - This work investigates the use of HPLC-SEC to characterise dissolved organic matter (DOM) of complex wastewater effluents. A silica-based column, sodium acetate eluent and multiple detections were employed: UV-254 absorbance for humictype, and tryptophan-like (Ex/Em = 270/355) and tyrosine-like (Ex/Em = 270/310) fluorescence for protein type compounds. Effects of eluent pH, eluent ionic strength and injection volume on separation efficiency were tested. Humic-type and protein-type fractions were clearly differentiated and eluted within and out of calibration range. Eluent ionic strength had the greatest influence on global resolution; the lowest eluent concentration of 0.01 M produced the best separation for all wastewater effluents tested at any detection. UV-254 absorbance was higher at neutral and basic eluent pH while tryptophan-like fluorescence depended on the sample composition rather than on the eluent pH or ionic strength. Tyrosine-like fluorescence decreased significantly with the increase of eluent ionic strength. Accurate molecular weight measurements could not be done, the separation being influenced by secondary interactions, but could be approximated using separate calibrations with sodium salts of polystyrene-sulfonates and protein standards. The results show that this method is suitable for determining DOM in wastewater at low eluent concentrations (up to 0.03 M), at neutral or slightly basic pH.

AB - This work investigates the use of HPLC-SEC to characterise dissolved organic matter (DOM) of complex wastewater effluents. A silica-based column, sodium acetate eluent and multiple detections were employed: UV-254 absorbance for humictype, and tryptophan-like (Ex/Em = 270/355) and tyrosine-like (Ex/Em = 270/310) fluorescence for protein type compounds. Effects of eluent pH, eluent ionic strength and injection volume on separation efficiency were tested. Humic-type and protein-type fractions were clearly differentiated and eluted within and out of calibration range. Eluent ionic strength had the greatest influence on global resolution; the lowest eluent concentration of 0.01 M produced the best separation for all wastewater effluents tested at any detection. UV-254 absorbance was higher at neutral and basic eluent pH while tryptophan-like fluorescence depended on the sample composition rather than on the eluent pH or ionic strength. Tyrosine-like fluorescence decreased significantly with the increase of eluent ionic strength. Accurate molecular weight measurements could not be done, the separation being influenced by secondary interactions, but could be approximated using separate calibrations with sodium salts of polystyrene-sulfonates and protein standards. The results show that this method is suitable for determining DOM in wastewater at low eluent concentrations (up to 0.03 M), at neutral or slightly basic pH.

KW - DOM

KW - Fluorescence

KW - greywater

KW - humic

KW - ionic strength

KW - proteins

KW - tryptophan

KW - UV-254

U2 - 10.1080/03067319.2016.1150463

DO - 10.1080/03067319.2016.1150463

M3 - Article

VL - 96

SP - 257

EP - 270

JO - International Journal of Environmental Analytical Chemistry

JF - International Journal of Environmental Analytical Chemistry

SN - 0306-7319

IS - 3

ER -