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Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds is a Potential Approach for Tendon Tissue Engineering

Tutkimustuotosvertaisarvioitu

Standard

Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds is a Potential Approach for Tendon Tissue Engineering. / Vuornos, Kaisa; Björninen, Miina; Talvitie, Elina; Paakinaho, Kaarlo; Kellomäki, Minna; Huhtala, Heini; Miettinen, Susanna; Seppänen-Kaijansinkko, Riitta; Haimi, Suvi.

julkaisussa: Tissue Engineering Part A, Vuosikerta 22, Nro 5-6, 01.03.2016, s. 513-523.

Tutkimustuotosvertaisarvioitu

Harvard

Vuornos, K, Björninen, M, Talvitie, E, Paakinaho, K, Kellomäki, M, Huhtala, H, Miettinen, S, Seppänen-Kaijansinkko, R & Haimi, S 2016, 'Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds is a Potential Approach for Tendon Tissue Engineering', Tissue Engineering Part A, Vuosikerta. 22, Nro 5-6, Sivut 513-523. https://doi.org/10.1089/ten.tea.2015.0276

APA

Vuornos, K., Björninen, M., Talvitie, E., Paakinaho, K., Kellomäki, M., Huhtala, H., ... Haimi, S. (2016). Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds is a Potential Approach for Tendon Tissue Engineering. Tissue Engineering Part A, 22(5-6), 513-523. https://doi.org/10.1089/ten.tea.2015.0276

Vancouver

Vuornos K, Björninen M, Talvitie E, Paakinaho K, Kellomäki M, Huhtala H et al. Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds is a Potential Approach for Tendon Tissue Engineering. Tissue Engineering Part A. 2016 maalis 1;22(5-6):513-523. https://doi.org/10.1089/ten.tea.2015.0276

Author

Vuornos, Kaisa ; Björninen, Miina ; Talvitie, Elina ; Paakinaho, Kaarlo ; Kellomäki, Minna ; Huhtala, Heini ; Miettinen, Susanna ; Seppänen-Kaijansinkko, Riitta ; Haimi, Suvi. / Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds is a Potential Approach for Tendon Tissue Engineering. Julkaisussa: Tissue Engineering Part A. 2016 ; Vuosikerta 22, Nro 5-6. Sivut 513-523.

Bibtex - Lataa

@article{07cca813429d4a58b445a06e51256cf8,
title = "Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds is a Potential Approach for Tendon Tissue Engineering",
abstract = "Growing number of musculoskeletal defects increases the demand for engineered tendon. Our aim was to find an efficient strategy to produce tendon-like matrix in vitro. To allow efficient differentiation of human adipose stem cells (hASCs) toward tendon tissue, we tested different medium compositions, biomaterials, and scaffold structures in preliminary tests. This is the first study to report that medium supplementation with 50 ng/mL of growth and differentiation factor-5 (GDF-5) and 280 μM l-ascorbic acid are essential for tenogenic differentiation of hASCs. Tenogenic medium (TM) was shown to significantly enhance tendon-like matrix production of hASCs compared to other tested media groups. Cell adhesion, proliferation, and tenogenic differentiation of hASCs were supported on braided poly(l/d)lactide (PLA) 96l/4d copolymer filament scaffolds in TM condition compared to foamed poly(l-lactide-co-ε-caprolactone) (PLCL) 70L/30CL scaffolds. A uniform cell layer formed on braided PLA 96/4 scaffolds when hASCs were cultured in TM compared to maintenance medium (MM) condition after 14 days of culture. Furthermore, total collagen content and gene expression of tenogenic marker genes were significantly higher in TM condition after 2 weeks of culture. The elastic modulus of PLA 96/4 scaffold was more similar to the elastic modulus reported for native Achilles tendon. Our study showed that the optimized TM is needed for efficient and rapid in vitro tenogenic extracellular matrix production of hASCs. PLA 96/4 scaffolds together with TM significantly stimulated hASCs, thus demonstrating the potential clinical relevance of this novel and emerging approach to tendon injury treatments in the future.",
author = "Kaisa Vuornos and Miina Bj{\"o}rninen and Elina Talvitie and Kaarlo Paakinaho and Minna Kellom{\"a}ki and Heini Huhtala and Susanna Miettinen and Riitta Sepp{\"a}nen-Kaijansinkko and Suvi Haimi",
note = "EXT={"}Vuornos, Kaisa{"}",
year = "2016",
month = "3",
day = "1",
doi = "10.1089/ten.tea.2015.0276",
language = "English",
volume = "22",
pages = "513--523",
journal = "Tissue Engineering Part A",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "5-6",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds is a Potential Approach for Tendon Tissue Engineering

AU - Vuornos, Kaisa

AU - Björninen, Miina

AU - Talvitie, Elina

AU - Paakinaho, Kaarlo

AU - Kellomäki, Minna

AU - Huhtala, Heini

AU - Miettinen, Susanna

AU - Seppänen-Kaijansinkko, Riitta

AU - Haimi, Suvi

N1 - EXT="Vuornos, Kaisa"

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Growing number of musculoskeletal defects increases the demand for engineered tendon. Our aim was to find an efficient strategy to produce tendon-like matrix in vitro. To allow efficient differentiation of human adipose stem cells (hASCs) toward tendon tissue, we tested different medium compositions, biomaterials, and scaffold structures in preliminary tests. This is the first study to report that medium supplementation with 50 ng/mL of growth and differentiation factor-5 (GDF-5) and 280 μM l-ascorbic acid are essential for tenogenic differentiation of hASCs. Tenogenic medium (TM) was shown to significantly enhance tendon-like matrix production of hASCs compared to other tested media groups. Cell adhesion, proliferation, and tenogenic differentiation of hASCs were supported on braided poly(l/d)lactide (PLA) 96l/4d copolymer filament scaffolds in TM condition compared to foamed poly(l-lactide-co-ε-caprolactone) (PLCL) 70L/30CL scaffolds. A uniform cell layer formed on braided PLA 96/4 scaffolds when hASCs were cultured in TM compared to maintenance medium (MM) condition after 14 days of culture. Furthermore, total collagen content and gene expression of tenogenic marker genes were significantly higher in TM condition after 2 weeks of culture. The elastic modulus of PLA 96/4 scaffold was more similar to the elastic modulus reported for native Achilles tendon. Our study showed that the optimized TM is needed for efficient and rapid in vitro tenogenic extracellular matrix production of hASCs. PLA 96/4 scaffolds together with TM significantly stimulated hASCs, thus demonstrating the potential clinical relevance of this novel and emerging approach to tendon injury treatments in the future.

AB - Growing number of musculoskeletal defects increases the demand for engineered tendon. Our aim was to find an efficient strategy to produce tendon-like matrix in vitro. To allow efficient differentiation of human adipose stem cells (hASCs) toward tendon tissue, we tested different medium compositions, biomaterials, and scaffold structures in preliminary tests. This is the first study to report that medium supplementation with 50 ng/mL of growth and differentiation factor-5 (GDF-5) and 280 μM l-ascorbic acid are essential for tenogenic differentiation of hASCs. Tenogenic medium (TM) was shown to significantly enhance tendon-like matrix production of hASCs compared to other tested media groups. Cell adhesion, proliferation, and tenogenic differentiation of hASCs were supported on braided poly(l/d)lactide (PLA) 96l/4d copolymer filament scaffolds in TM condition compared to foamed poly(l-lactide-co-ε-caprolactone) (PLCL) 70L/30CL scaffolds. A uniform cell layer formed on braided PLA 96/4 scaffolds when hASCs were cultured in TM compared to maintenance medium (MM) condition after 14 days of culture. Furthermore, total collagen content and gene expression of tenogenic marker genes were significantly higher in TM condition after 2 weeks of culture. The elastic modulus of PLA 96/4 scaffold was more similar to the elastic modulus reported for native Achilles tendon. Our study showed that the optimized TM is needed for efficient and rapid in vitro tenogenic extracellular matrix production of hASCs. PLA 96/4 scaffolds together with TM significantly stimulated hASCs, thus demonstrating the potential clinical relevance of this novel and emerging approach to tendon injury treatments in the future.

U2 - 10.1089/ten.tea.2015.0276

DO - 10.1089/ten.tea.2015.0276

M3 - Article

VL - 22

SP - 513

EP - 523

JO - Tissue Engineering Part A

JF - Tissue Engineering Part A

SN - 1937-3341

IS - 5-6

ER -