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Human adipose stem cells in chondrogenic differentiation medium without growth factors differentiate towards annulus fibrosus phenotype in vitro

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Human adipose stem cells in chondrogenic differentiation medium without growth factors differentiate towards annulus fibrosus phenotype in vitro. / Gebraad, A. W H; Miettinen, S.; Grijpma, D. W.; Haimi, S. P.

julkaisussa: Macromolecular symposia, Vuosikerta 334, Nro 1, 12.2013, s. 49-56.

Tutkimustuotosvertaisarvioitu

Harvard

Gebraad, AWH, Miettinen, S, Grijpma, DW & Haimi, SP 2013, 'Human adipose stem cells in chondrogenic differentiation medium without growth factors differentiate towards annulus fibrosus phenotype in vitro', Macromolecular symposia, Vuosikerta. 334, Nro 1, Sivut 49-56. https://doi.org/10.1002/masy.201300104

APA

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Author

Gebraad, A. W H ; Miettinen, S. ; Grijpma, D. W. ; Haimi, S. P. / Human adipose stem cells in chondrogenic differentiation medium without growth factors differentiate towards annulus fibrosus phenotype in vitro. Julkaisussa: Macromolecular symposia. 2013 ; Vuosikerta 334, Nro 1. Sivut 49-56.

Bibtex - Lataa

@article{7966f83e54b14dc68e9e097e060bc551,
title = "Human adipose stem cells in chondrogenic differentiation medium without growth factors differentiate towards annulus fibrosus phenotype in vitro",
abstract = "Summary Intervertebral disc degeneration is the main cause of chronic back pain. Disc degeneration mainly leads to tearing of annulus fibrosus (AF), which is with current methods difficult to restore and impossible to regenerate. Stem cell technology offers a potential technique to repair the ruptured AF by enabling new matrix synthesis at the defect site. Previous studies have shown that human adipose stem cells (hASCs) are able to differentiate towards AF phenotype when treated with suitable growth factors. There are concerns about the use of growth factors in clinical applications because of their short half-lives, high costs and low effectiveness. The main aim of this research project was to regenerate AF tissue in vitro using hASCs and serum free chondrogenic medium without supplementation of growth factors. Differentiation of hASCs was induced by using the micromass culture technique. Human annulus fibrosus cell (hAFCs) cultured in commercial AF growth medium were used as positive control. Assessment of AF phenotype of hASCs and hAFCs was done at 14 and 21 days of culture. Quantification of sulphated glycosaminoglycan (GAG) content showed that hASCs cultured in chondrogenic medium expressed similar levels of sulphated GAGs as hAFCs. qRT-PCR confirmed the similarity of the differentiated hASCs with AF phenotype. Several markers for AF phenotype (aggrecan, collagen type I and glypican-3) were expressed in both hAFCs and differentiated hASCs. This is the first study that demonstrated that hASCs can be differentiated towards AF phenotype using serum free chondrogenic medium without growth factors. In a next step, scaffolds manufactured from biodegradable polymers will be used in combination with ASCs to find an optimal construct to repair AF defects.",
keywords = "adipose stem cells, annulus fibrosus, intervertebral disc, tissue engineering",
author = "Gebraad, {A. W H} and S. Miettinen and Grijpma, {D. W.} and Haimi, {S. P.}",
year = "2013",
month = "12",
doi = "10.1002/masy.201300104",
language = "English",
volume = "334",
pages = "49--56",
journal = "Macromolecular symposia",
issn = "1022-1360",
publisher = "Wiley-VCH Verlag",
number = "1",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Human adipose stem cells in chondrogenic differentiation medium without growth factors differentiate towards annulus fibrosus phenotype in vitro

AU - Gebraad, A. W H

AU - Miettinen, S.

AU - Grijpma, D. W.

AU - Haimi, S. P.

PY - 2013/12

Y1 - 2013/12

N2 - Summary Intervertebral disc degeneration is the main cause of chronic back pain. Disc degeneration mainly leads to tearing of annulus fibrosus (AF), which is with current methods difficult to restore and impossible to regenerate. Stem cell technology offers a potential technique to repair the ruptured AF by enabling new matrix synthesis at the defect site. Previous studies have shown that human adipose stem cells (hASCs) are able to differentiate towards AF phenotype when treated with suitable growth factors. There are concerns about the use of growth factors in clinical applications because of their short half-lives, high costs and low effectiveness. The main aim of this research project was to regenerate AF tissue in vitro using hASCs and serum free chondrogenic medium without supplementation of growth factors. Differentiation of hASCs was induced by using the micromass culture technique. Human annulus fibrosus cell (hAFCs) cultured in commercial AF growth medium were used as positive control. Assessment of AF phenotype of hASCs and hAFCs was done at 14 and 21 days of culture. Quantification of sulphated glycosaminoglycan (GAG) content showed that hASCs cultured in chondrogenic medium expressed similar levels of sulphated GAGs as hAFCs. qRT-PCR confirmed the similarity of the differentiated hASCs with AF phenotype. Several markers for AF phenotype (aggrecan, collagen type I and glypican-3) were expressed in both hAFCs and differentiated hASCs. This is the first study that demonstrated that hASCs can be differentiated towards AF phenotype using serum free chondrogenic medium without growth factors. In a next step, scaffolds manufactured from biodegradable polymers will be used in combination with ASCs to find an optimal construct to repair AF defects.

AB - Summary Intervertebral disc degeneration is the main cause of chronic back pain. Disc degeneration mainly leads to tearing of annulus fibrosus (AF), which is with current methods difficult to restore and impossible to regenerate. Stem cell technology offers a potential technique to repair the ruptured AF by enabling new matrix synthesis at the defect site. Previous studies have shown that human adipose stem cells (hASCs) are able to differentiate towards AF phenotype when treated with suitable growth factors. There are concerns about the use of growth factors in clinical applications because of their short half-lives, high costs and low effectiveness. The main aim of this research project was to regenerate AF tissue in vitro using hASCs and serum free chondrogenic medium without supplementation of growth factors. Differentiation of hASCs was induced by using the micromass culture technique. Human annulus fibrosus cell (hAFCs) cultured in commercial AF growth medium were used as positive control. Assessment of AF phenotype of hASCs and hAFCs was done at 14 and 21 days of culture. Quantification of sulphated glycosaminoglycan (GAG) content showed that hASCs cultured in chondrogenic medium expressed similar levels of sulphated GAGs as hAFCs. qRT-PCR confirmed the similarity of the differentiated hASCs with AF phenotype. Several markers for AF phenotype (aggrecan, collagen type I and glypican-3) were expressed in both hAFCs and differentiated hASCs. This is the first study that demonstrated that hASCs can be differentiated towards AF phenotype using serum free chondrogenic medium without growth factors. In a next step, scaffolds manufactured from biodegradable polymers will be used in combination with ASCs to find an optimal construct to repair AF defects.

KW - adipose stem cells

KW - annulus fibrosus

KW - intervertebral disc

KW - tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84890722713&partnerID=8YFLogxK

U2 - 10.1002/masy.201300104

DO - 10.1002/masy.201300104

M3 - Article

VL - 334

SP - 49

EP - 56

JO - Macromolecular symposia

JF - Macromolecular symposia

SN - 1022-1360

IS - 1

ER -