TUTCRIS - Tampereen teknillinen yliopisto

TUTCRIS

Identification of single microbial particles using electro-dynamic balance assisted laser-induced breakdown and fluorescence spectroscopy

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut126-132
Sivumäärä7
JulkaisuAerosol Science and Technology
Vuosikerta50
Numero2
DOI - pysyväislinkit
TilaJulkaistu - 1 helmikuuta 2016
OKM-julkaisutyyppiA1 Alkuperäisartikkeli

Tiivistelmä

Online characterization of fungal and bacterial spores is important in various applications due to their health and climatic relevance. The aim of this study was to demonstrate the capability of the combination of electro-dynamic balance assisted laser-induced breakdown spectroscopy (LIBS) and laser-induced fluorescence (LIF) techniques for the online detection of single fungal spores (Aspergillus versicolor and Penicillium brevicompactum) and bacteria (Bacillus aureus). The method enabled sensitive and repeatable LIBS analysis of common elemental components (Ca, Na, and K) from single microbial particles for the first time. Significant differences in the concentrations of these elements were observed between the species, e.g., bacterial spores had over three orders of magnitude higher Ca concentration (2 × 10-12 g/particle) compared to fungal spores (3-5 × 10-16 g/particle). The LIF analysis has previously been used to distinguish bioaerosols from other aerosols due to their fluorescence ability. This study showed that combination of LIF and LIBS analysis is a promising tool for identification of different bioaerosol particle types.

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