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Improved bioconversion of crude glycerol to hydrogen by statistical optimization of media components

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Improved bioconversion of crude glycerol to hydrogen by statistical optimization of media components. / Mangayil, Rahul; Aho, Tommi; Karp, Matti; Santala, Ville.

julkaisussa: Renewable Energy, Vuosikerta 75, 01.03.2015, s. 583-589.

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Mangayil, Rahul ; Aho, Tommi ; Karp, Matti ; Santala, Ville. / Improved bioconversion of crude glycerol to hydrogen by statistical optimization of media components. Julkaisussa: Renewable Energy. 2015 ; Vuosikerta 75. Sivut 583-589.

Bibtex - Lataa

@article{049d5e4b9d6f4a1fa8323001c851d910,
title = "Improved bioconversion of crude glycerol to hydrogen by statistical optimization of media components",
abstract = "Bioconversion of crude glycerol to hydrogen has gained importance as it addresses both sustainable energy production and waste disposal issues. Until recently, statistical optimizations of crude glycerol bioconversion to hydrogen have been greatly focused on pure strains. In this study, biohydrogen production from crude glycerol by an enriched microbial culture (predominated with Clostridium species) was improved by statistical optimization of media components. Plackett-Burman design identified MgCl2.6H2O and KCl with negative effect on hydrogen production and selected NH4Cl, K2HPO4 and KH2PO4 as significant variables. Box-Behnken design indicated the optimal region beyond design area and studies were continued by ridge analysis. Central composite face centered design envisaged a maximal hydrogen yield of 1.41mol-H2/mol-glycerolconsumed at concentrations 4.40g/L and 2.27g/L for NH4Cl and KH2PO4 respectively. Confirmation experiment with the optimized media (NH4Cl, 4.40g/L; K2HPO4, 1.6g/L; KH2PO4, 2.27g/L; MgCl2.6H2O, 1.0g/L; KCl, 1.0g/L; Na-acetate.3H2O, 1.0g/L and tryptone, 2.0g/L) revealed an excellent correlation between predicted and experimental hydrogen yield. Optimization of media components by design of experiments enhanced hydrogen yield by 29{\%}.",
keywords = "Biohydrogen, Crude glycerol, Optimization, Response surface methodology",
author = "Rahul Mangayil and Tommi Aho and Matti Karp and Ville Santala",
note = "Available online 3 November 2014 : Volume 75, March 2015, Pages 583-589<br/>Contribution: organisation=keb,FACT1=1<br/>Portfolio EDEND: 2014-12-12<br/>Publisher name: Pergamon; The World Renewable Energy Network",
year = "2015",
month = "3",
day = "1",
doi = "10.1016/j.renene.2014.10.051",
language = "English",
volume = "75",
pages = "583--589",
journal = "Renewable Energy",
issn = "0960-1481",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Improved bioconversion of crude glycerol to hydrogen by statistical optimization of media components

AU - Mangayil, Rahul

AU - Aho, Tommi

AU - Karp, Matti

AU - Santala, Ville

N1 - Available online 3 November 2014 : Volume 75, March 2015, Pages 583-589<br/>Contribution: organisation=keb,FACT1=1<br/>Portfolio EDEND: 2014-12-12<br/>Publisher name: Pergamon; The World Renewable Energy Network

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Bioconversion of crude glycerol to hydrogen has gained importance as it addresses both sustainable energy production and waste disposal issues. Until recently, statistical optimizations of crude glycerol bioconversion to hydrogen have been greatly focused on pure strains. In this study, biohydrogen production from crude glycerol by an enriched microbial culture (predominated with Clostridium species) was improved by statistical optimization of media components. Plackett-Burman design identified MgCl2.6H2O and KCl with negative effect on hydrogen production and selected NH4Cl, K2HPO4 and KH2PO4 as significant variables. Box-Behnken design indicated the optimal region beyond design area and studies were continued by ridge analysis. Central composite face centered design envisaged a maximal hydrogen yield of 1.41mol-H2/mol-glycerolconsumed at concentrations 4.40g/L and 2.27g/L for NH4Cl and KH2PO4 respectively. Confirmation experiment with the optimized media (NH4Cl, 4.40g/L; K2HPO4, 1.6g/L; KH2PO4, 2.27g/L; MgCl2.6H2O, 1.0g/L; KCl, 1.0g/L; Na-acetate.3H2O, 1.0g/L and tryptone, 2.0g/L) revealed an excellent correlation between predicted and experimental hydrogen yield. Optimization of media components by design of experiments enhanced hydrogen yield by 29%.

AB - Bioconversion of crude glycerol to hydrogen has gained importance as it addresses both sustainable energy production and waste disposal issues. Until recently, statistical optimizations of crude glycerol bioconversion to hydrogen have been greatly focused on pure strains. In this study, biohydrogen production from crude glycerol by an enriched microbial culture (predominated with Clostridium species) was improved by statistical optimization of media components. Plackett-Burman design identified MgCl2.6H2O and KCl with negative effect on hydrogen production and selected NH4Cl, K2HPO4 and KH2PO4 as significant variables. Box-Behnken design indicated the optimal region beyond design area and studies were continued by ridge analysis. Central composite face centered design envisaged a maximal hydrogen yield of 1.41mol-H2/mol-glycerolconsumed at concentrations 4.40g/L and 2.27g/L for NH4Cl and KH2PO4 respectively. Confirmation experiment with the optimized media (NH4Cl, 4.40g/L; K2HPO4, 1.6g/L; KH2PO4, 2.27g/L; MgCl2.6H2O, 1.0g/L; KCl, 1.0g/L; Na-acetate.3H2O, 1.0g/L and tryptone, 2.0g/L) revealed an excellent correlation between predicted and experimental hydrogen yield. Optimization of media components by design of experiments enhanced hydrogen yield by 29%.

KW - Biohydrogen

KW - Crude glycerol

KW - Optimization

KW - Response surface methodology

UR - http://www.scopus.com/inward/record.url?scp=84910051633&partnerID=8YFLogxK

U2 - 10.1016/j.renene.2014.10.051

DO - 10.1016/j.renene.2014.10.051

M3 - Article

VL - 75

SP - 583

EP - 589

JO - Renewable Energy

JF - Renewable Energy

SN - 0960-1481

ER -