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In vitro bioluminescence used as a method for real-time inhibition zone testing for antibiotic-releasing composites

Tutkimustuotosvertaisarvioitu

Standard

In vitro bioluminescence used as a method for real-time inhibition zone testing for antibiotic-releasing composites. / Männistö, Noora M.; Ahola, Niina; Karp, Matti T.; Veiranto, Minna; Kellomäki, Minna.

julkaisussa: British Microbiology Research Journal, Vuosikerta 4, Nro 2, 2014, s. 235-254.

Tutkimustuotosvertaisarvioitu

Harvard

Männistö, NM, Ahola, N, Karp, MT, Veiranto, M & Kellomäki, M 2014, 'In vitro bioluminescence used as a method for real-time inhibition zone testing for antibiotic-releasing composites', British Microbiology Research Journal, Vuosikerta. 4, Nro 2, Sivut 235-254. https://doi.org/10.9734/BMRJ/2014/6661

APA

Männistö, N. M., Ahola, N., Karp, M. T., Veiranto, M., & Kellomäki, M. (2014). In vitro bioluminescence used as a method for real-time inhibition zone testing for antibiotic-releasing composites. British Microbiology Research Journal, 4(2), 235-254. https://doi.org/10.9734/BMRJ/2014/6661

Vancouver

Author

Männistö, Noora M. ; Ahola, Niina ; Karp, Matti T. ; Veiranto, Minna ; Kellomäki, Minna. / In vitro bioluminescence used as a method for real-time inhibition zone testing for antibiotic-releasing composites. Julkaisussa: British Microbiology Research Journal. 2014 ; Vuosikerta 4, Nro 2. Sivut 235-254.

Bibtex - Lataa

@article{41955315577b4add8929ecc279a34b86,
title = "In vitro bioluminescence used as a method for real-time inhibition zone testing for antibiotic-releasing composites",
abstract = "Aims: This study describes the potential of real-time bioluminescence imaging in evaluating the antibiotic efficiency of two cylinder-shaped bioabsorbable antibiotic-releasing composites by in vitro inhibition zone tests. The bacterial infections of bone tissue can cause extensive hard and soft tissue damage and decrease the efficiency of oral antibiotic therapy due to the poor blood circulation in the infected area. To overcome this problem, new, locally antibiotic-releasing biodegradable composites have been developed. Study Design & Methodology: The two composites evaluated in this study were composed of poly(L-lactide-co-ε-caprolactone) matrix, β-tricalcium phosphate ceramic and either ciprofloxacin or rifampicin antibiotic. The composites were tested with genetically modified model pathogens of osteomyelitis (Pseudomonas aeruginosa and Staphylococcus epidermidis) in vitro in inhibition zone tests using a method of real-time bioluminescence. Results: The first signs of the effect of the released ciprofloxacin or rifampicin became visible after four hours of incubation and were seen as changed bioluminescence around the composite pellet on a culture dish. Both of the composite types showed excellent effects against the sensor bacteria within the diffusion area. Bioluminescence measurements suggested that no survivor bacteria capable of evolving resistant strains were left inside the inhibition zones. The S. epidermidis bacterial strain was an inhibition sensor and P. aeruginosa was a stress sensor. Conclusion: These results highlight the potential of the composite materials against the pathogens of osteomyelitis. The approach allows continuous visual inspection of the efficacy of the antibiotics against the bacteria.",
author = "M{\"a}nnist{\"o}, {Noora M.} and Niina Ahola and Karp, {Matti T.} and Minna Veiranto and Minna Kellom{\"a}ki",
note = "Contribution: organisation=keb,FACT1=0.5<br/>Contribution: organisation=elt,FACT2=0.5<br/>Portfolio EDEND: 2014-02-15<br/>Publisher name: Sciencedomain International",
year = "2014",
doi = "10.9734/BMRJ/2014/6661",
language = "English",
volume = "4",
pages = "235--254",
journal = "British Microbiology Research Journal",
issn = "2231-0886",
publisher = "SCIENCEDOMAIN International",
number = "2",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - In vitro bioluminescence used as a method for real-time inhibition zone testing for antibiotic-releasing composites

AU - Männistö, Noora M.

AU - Ahola, Niina

AU - Karp, Matti T.

AU - Veiranto, Minna

AU - Kellomäki, Minna

N1 - Contribution: organisation=keb,FACT1=0.5<br/>Contribution: organisation=elt,FACT2=0.5<br/>Portfolio EDEND: 2014-02-15<br/>Publisher name: Sciencedomain International

PY - 2014

Y1 - 2014

N2 - Aims: This study describes the potential of real-time bioluminescence imaging in evaluating the antibiotic efficiency of two cylinder-shaped bioabsorbable antibiotic-releasing composites by in vitro inhibition zone tests. The bacterial infections of bone tissue can cause extensive hard and soft tissue damage and decrease the efficiency of oral antibiotic therapy due to the poor blood circulation in the infected area. To overcome this problem, new, locally antibiotic-releasing biodegradable composites have been developed. Study Design & Methodology: The two composites evaluated in this study were composed of poly(L-lactide-co-ε-caprolactone) matrix, β-tricalcium phosphate ceramic and either ciprofloxacin or rifampicin antibiotic. The composites were tested with genetically modified model pathogens of osteomyelitis (Pseudomonas aeruginosa and Staphylococcus epidermidis) in vitro in inhibition zone tests using a method of real-time bioluminescence. Results: The first signs of the effect of the released ciprofloxacin or rifampicin became visible after four hours of incubation and were seen as changed bioluminescence around the composite pellet on a culture dish. Both of the composite types showed excellent effects against the sensor bacteria within the diffusion area. Bioluminescence measurements suggested that no survivor bacteria capable of evolving resistant strains were left inside the inhibition zones. The S. epidermidis bacterial strain was an inhibition sensor and P. aeruginosa was a stress sensor. Conclusion: These results highlight the potential of the composite materials against the pathogens of osteomyelitis. The approach allows continuous visual inspection of the efficacy of the antibiotics against the bacteria.

AB - Aims: This study describes the potential of real-time bioluminescence imaging in evaluating the antibiotic efficiency of two cylinder-shaped bioabsorbable antibiotic-releasing composites by in vitro inhibition zone tests. The bacterial infections of bone tissue can cause extensive hard and soft tissue damage and decrease the efficiency of oral antibiotic therapy due to the poor blood circulation in the infected area. To overcome this problem, new, locally antibiotic-releasing biodegradable composites have been developed. Study Design & Methodology: The two composites evaluated in this study were composed of poly(L-lactide-co-ε-caprolactone) matrix, β-tricalcium phosphate ceramic and either ciprofloxacin or rifampicin antibiotic. The composites were tested with genetically modified model pathogens of osteomyelitis (Pseudomonas aeruginosa and Staphylococcus epidermidis) in vitro in inhibition zone tests using a method of real-time bioluminescence. Results: The first signs of the effect of the released ciprofloxacin or rifampicin became visible after four hours of incubation and were seen as changed bioluminescence around the composite pellet on a culture dish. Both of the composite types showed excellent effects against the sensor bacteria within the diffusion area. Bioluminescence measurements suggested that no survivor bacteria capable of evolving resistant strains were left inside the inhibition zones. The S. epidermidis bacterial strain was an inhibition sensor and P. aeruginosa was a stress sensor. Conclusion: These results highlight the potential of the composite materials against the pathogens of osteomyelitis. The approach allows continuous visual inspection of the efficacy of the antibiotics against the bacteria.

U2 - 10.9734/BMRJ/2014/6661

DO - 10.9734/BMRJ/2014/6661

M3 - Article

VL - 4

SP - 235

EP - 254

JO - British Microbiology Research Journal

JF - British Microbiology Research Journal

SN - 2231-0886

IS - 2

ER -