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Influence of electron acceptor availability and microbial community structure on sedimentary methane oxidation in a boreal estuary

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Influence of electron acceptor availability and microbial community structure on sedimentary methane oxidation in a boreal estuary. / Myllykangas, Jukka Pekka; Rissanen, Antti J.; Hietanen, Susanna; Jilbert, Tom.

julkaisussa: BIOGEOCHEMISTRY, Vuosikerta 148, Nro 3, 2020, s. 291-309.

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Myllykangas, Jukka Pekka ; Rissanen, Antti J. ; Hietanen, Susanna ; Jilbert, Tom. / Influence of electron acceptor availability and microbial community structure on sedimentary methane oxidation in a boreal estuary. Julkaisussa: BIOGEOCHEMISTRY. 2020 ; Vuosikerta 148, Nro 3. Sivut 291-309.

Bibtex - Lataa

@article{8be065272d17475bb6feda062b5644d3,
title = "Influence of electron acceptor availability and microbial community structure on sedimentary methane oxidation in a boreal estuary",
abstract = "Methane is produced microbially in vast quantities in sediments throughout the world’s oceans. However, anaerobic oxidation of methane (AOM) provides a near-quantitative sink for the produced methane and is primarily responsible for preventing methane emissions from the oceans to the atmosphere. AOM is a complex microbial process that involves several different microbial groups and metabolic pathways. The role of different electron acceptors in AOM has been studied for decades, yet large uncertainties remain, especially in terms of understanding the processes in natural settings. This study reports whole-core incubation methane oxidation rates along an estuarine gradient ranging from near fresh water to brackish conditions, and investigates the potential role of different electron acceptors in AOM. Microbial community structure involved in different methane processes is also studied in the same estuarine system using high throughput sequencing tools. Methane oxidation in the sediments was active in three distinct depth layers throughout the studied transect, with total oxidation rates increasing seawards. We find extensive evidence of non-sulphate AOM throughout the transect. The highest absolute AOM rates were observed below the sulphate-methane transition zone (SMTZ), strongly implicating the role of alternative electron acceptors (most likely iron and manganese oxides). However, oxidation rates were ultimately limited by methane availability. ANME-2a/b were the most abundant microbial phyla associated with AOM throughout the study sites, followed by ANME-2d in much lower abundances. Similarly to oxidation rates, highest abundances of microbial groups commonly associated with AOM were found well below the SMTZ, further reinforcing the importance of non-sulphate AOM in this system.",
keywords = "16S rRNA gene, Baltic sea, High throughput sequencing, Methanotrophy, Radiotracer incubation",
author = "Myllykangas, {Jukka Pekka} and Rissanen, {Antti J.} and Susanna Hietanen and Tom Jilbert",
year = "2020",
doi = "10.1007/s10533-020-00660-z",
language = "English",
volume = "148",
pages = "291--309",
journal = "BIOGEOCHEMISTRY",
issn = "0168-2563",
publisher = "Springer Verlag",
number = "3",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Influence of electron acceptor availability and microbial community structure on sedimentary methane oxidation in a boreal estuary

AU - Myllykangas, Jukka Pekka

AU - Rissanen, Antti J.

AU - Hietanen, Susanna

AU - Jilbert, Tom

PY - 2020

Y1 - 2020

N2 - Methane is produced microbially in vast quantities in sediments throughout the world’s oceans. However, anaerobic oxidation of methane (AOM) provides a near-quantitative sink for the produced methane and is primarily responsible for preventing methane emissions from the oceans to the atmosphere. AOM is a complex microbial process that involves several different microbial groups and metabolic pathways. The role of different electron acceptors in AOM has been studied for decades, yet large uncertainties remain, especially in terms of understanding the processes in natural settings. This study reports whole-core incubation methane oxidation rates along an estuarine gradient ranging from near fresh water to brackish conditions, and investigates the potential role of different electron acceptors in AOM. Microbial community structure involved in different methane processes is also studied in the same estuarine system using high throughput sequencing tools. Methane oxidation in the sediments was active in three distinct depth layers throughout the studied transect, with total oxidation rates increasing seawards. We find extensive evidence of non-sulphate AOM throughout the transect. The highest absolute AOM rates were observed below the sulphate-methane transition zone (SMTZ), strongly implicating the role of alternative electron acceptors (most likely iron and manganese oxides). However, oxidation rates were ultimately limited by methane availability. ANME-2a/b were the most abundant microbial phyla associated with AOM throughout the study sites, followed by ANME-2d in much lower abundances. Similarly to oxidation rates, highest abundances of microbial groups commonly associated with AOM were found well below the SMTZ, further reinforcing the importance of non-sulphate AOM in this system.

AB - Methane is produced microbially in vast quantities in sediments throughout the world’s oceans. However, anaerobic oxidation of methane (AOM) provides a near-quantitative sink for the produced methane and is primarily responsible for preventing methane emissions from the oceans to the atmosphere. AOM is a complex microbial process that involves several different microbial groups and metabolic pathways. The role of different electron acceptors in AOM has been studied for decades, yet large uncertainties remain, especially in terms of understanding the processes in natural settings. This study reports whole-core incubation methane oxidation rates along an estuarine gradient ranging from near fresh water to brackish conditions, and investigates the potential role of different electron acceptors in AOM. Microbial community structure involved in different methane processes is also studied in the same estuarine system using high throughput sequencing tools. Methane oxidation in the sediments was active in three distinct depth layers throughout the studied transect, with total oxidation rates increasing seawards. We find extensive evidence of non-sulphate AOM throughout the transect. The highest absolute AOM rates were observed below the sulphate-methane transition zone (SMTZ), strongly implicating the role of alternative electron acceptors (most likely iron and manganese oxides). However, oxidation rates were ultimately limited by methane availability. ANME-2a/b were the most abundant microbial phyla associated with AOM throughout the study sites, followed by ANME-2d in much lower abundances. Similarly to oxidation rates, highest abundances of microbial groups commonly associated with AOM were found well below the SMTZ, further reinforcing the importance of non-sulphate AOM in this system.

KW - 16S rRNA gene

KW - Baltic sea

KW - High throughput sequencing

KW - Methanotrophy

KW - Radiotracer incubation

U2 - 10.1007/s10533-020-00660-z

DO - 10.1007/s10533-020-00660-z

M3 - Article

VL - 148

SP - 291

EP - 309

JO - BIOGEOCHEMISTRY

JF - BIOGEOCHEMISTRY

SN - 0168-2563

IS - 3

ER -