TUTCRIS - Tampereen teknillinen yliopisto


Investigation of small molecule interaction with receptors in cancer therapy

Tutkimustuotos: Konferenssiesitys, posteri tai abstrakti


TilaJulkaistu - 27 syyskuuta 2017
OKM-julkaisutyyppiEi OKM-tyyppiä
TapahtumaCell Signaling, Cell Therapy & Cancer Therapeutics - Chicago, Chicago, Yhdysvallat
Kesto: 27 syyskuuta 201728 syyskuuta 2017
Konferenssinumero: 1249


ConferenceCell Signaling, Cell Therapy & Cancer Therapeutics


Cancer is a generic term, a second leading death causing disease, follows uncontrolled growth of abnormal cells results in death. The death rate is about to increase up to 23.3% in 2020, of that breast cancer ranks fourth. The western women suffer mostly with breast cancer than any other cancers. Plant-derived, marine-derived and synthetic phenolic compounds have been proposed as effective inhibitory agent for their anti-cancer activity. In our present work, we have screened the compound alkylaminophenols for their anti-cancerous property. Earlier studies of alkylaminophenols in Human osteosarcoma cell lines (U2OS) and Human embryonic kidney cell lines (HEK293T) results in a promising chemo-therapeutic effect. In our current study, we have chosen a receptor specific cell line, MCF-7 breast cell lines, through docking, a ligand-receptor interaction study. The preliminary studies on
the effect of cytotoxicity in alkylaminophenols, inhibits the cell growth at the range of 1-2μM (IC50). Further studies on DNA content morphology analysis and apoptosis protein expression analysis, we identified that alkylaminophenols can efficiently inhibit the growth of MCF-7 cell lines. Also, the cell death transition mechanism is also studied using the fluorescence microscope and fluorescence-
based assays were used to analyse the morphological variations at single cell level. Overall, we will discuss the cytotoxicity effect of alkylaminophenol and its mode of regulation at the single cell level and further studies are warranted to determine the gene expression profiling of these phenolic compounds.