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LTCC-Based Multi-Electrode Arrays for 3D in Vitro Cell Cultures

Tutkimustuotosvertaisarvioitu

Standard

LTCC-Based Multi-Electrode Arrays for 3D in Vitro Cell Cultures. / Bartsch, H.; Himmerlich, M.; Fischer, M.; Demko, L.; Hyttinen, J.; Schober, A.

julkaisussa: Journal of Ceramic Science and Technology, Vuosikerta 6, Nro 4, 12.2015, s. 315-324.

Tutkimustuotosvertaisarvioitu

Harvard

Bartsch, H, Himmerlich, M, Fischer, M, Demko, L, Hyttinen, J & Schober, A 2015, 'LTCC-Based Multi-Electrode Arrays for 3D in Vitro Cell Cultures', Journal of Ceramic Science and Technology, Vuosikerta. 6, Nro 4, Sivut 315-324. https://doi.org/10.4416/JCST2015-00056

APA

Bartsch, H., Himmerlich, M., Fischer, M., Demko, L., Hyttinen, J., & Schober, A. (2015). LTCC-Based Multi-Electrode Arrays for 3D in Vitro Cell Cultures. Journal of Ceramic Science and Technology, 6(4), 315-324. https://doi.org/10.4416/JCST2015-00056

Vancouver

Bartsch H, Himmerlich M, Fischer M, Demko L, Hyttinen J, Schober A. LTCC-Based Multi-Electrode Arrays for 3D in Vitro Cell Cultures. Journal of Ceramic Science and Technology. 2015 joulu;6(4):315-324. https://doi.org/10.4416/JCST2015-00056

Author

Bartsch, H. ; Himmerlich, M. ; Fischer, M. ; Demko, L. ; Hyttinen, J. ; Schober, A. / LTCC-Based Multi-Electrode Arrays for 3D in Vitro Cell Cultures. Julkaisussa: Journal of Ceramic Science and Technology. 2015 ; Vuosikerta 6, Nro 4. Sivut 315-324.

Bibtex - Lataa

@article{88b956f8d23c47e1ba1a206ab8b110a2,
title = "LTCC-Based Multi-Electrode Arrays for 3D in Vitro Cell Cultures",
abstract = "Current technologies to monitor neuronal cultures in vitro are based on 2-dimensional (2D) multi-electrode arrays and cell cultures. The complexity of actual high-level neurobiological systems requires 3-dimensional (3D) cultures and 3D electrode arrays to improve our understanding of such systems. The realization calls for smart multilayer and packaging technology. Our approach uses low-temperature cofired ceramics (LTCC) for the design of a 3-dimensional multi-electrode array (3D MEA). An LTCC multilayer board with gold electrodes forms the basis of the system. The layout of the 3D MEA is designed to fit into widely used measurement adapters for 2D signal recordings, enabling data processing identical to that of established chips. Design and manufacturing of the new 3D device as a basic tool for the investigation of 3D cell cultures are described. Features of thick-film gold electrodes are characterized by means of microscopic and spectroscopic tools complemented with complex impedance measurements. Possible biological applications for in vitro electrophysiological measurements were evaluated based on cell cultures of primary neurons, seeded directly to the chip surface. It was shown that activity can be measured over six months.",
keywords = "Low-temperature cofired ceramics (LTCC), three-dimensional hybrid multi-electrode array (3D MEA), in vitro cell culture, thick-film gold electrode, primary neuron culture, GROWTH",
author = "H. Bartsch and M. Himmerlich and M. Fischer and L. Demko and J. Hyttinen and A. Schober",
year = "2015",
month = "12",
doi = "10.4416/JCST2015-00056",
language = "English",
volume = "6",
pages = "315--324",
journal = "Journal of Ceramic Science and Technology",
issn = "2190-9385",
publisher = "GOLLER VERLAG GMBH",
number = "4",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - LTCC-Based Multi-Electrode Arrays for 3D in Vitro Cell Cultures

AU - Bartsch, H.

AU - Himmerlich, M.

AU - Fischer, M.

AU - Demko, L.

AU - Hyttinen, J.

AU - Schober, A.

PY - 2015/12

Y1 - 2015/12

N2 - Current technologies to monitor neuronal cultures in vitro are based on 2-dimensional (2D) multi-electrode arrays and cell cultures. The complexity of actual high-level neurobiological systems requires 3-dimensional (3D) cultures and 3D electrode arrays to improve our understanding of such systems. The realization calls for smart multilayer and packaging technology. Our approach uses low-temperature cofired ceramics (LTCC) for the design of a 3-dimensional multi-electrode array (3D MEA). An LTCC multilayer board with gold electrodes forms the basis of the system. The layout of the 3D MEA is designed to fit into widely used measurement adapters for 2D signal recordings, enabling data processing identical to that of established chips. Design and manufacturing of the new 3D device as a basic tool for the investigation of 3D cell cultures are described. Features of thick-film gold electrodes are characterized by means of microscopic and spectroscopic tools complemented with complex impedance measurements. Possible biological applications for in vitro electrophysiological measurements were evaluated based on cell cultures of primary neurons, seeded directly to the chip surface. It was shown that activity can be measured over six months.

AB - Current technologies to monitor neuronal cultures in vitro are based on 2-dimensional (2D) multi-electrode arrays and cell cultures. The complexity of actual high-level neurobiological systems requires 3-dimensional (3D) cultures and 3D electrode arrays to improve our understanding of such systems. The realization calls for smart multilayer and packaging technology. Our approach uses low-temperature cofired ceramics (LTCC) for the design of a 3-dimensional multi-electrode array (3D MEA). An LTCC multilayer board with gold electrodes forms the basis of the system. The layout of the 3D MEA is designed to fit into widely used measurement adapters for 2D signal recordings, enabling data processing identical to that of established chips. Design and manufacturing of the new 3D device as a basic tool for the investigation of 3D cell cultures are described. Features of thick-film gold electrodes are characterized by means of microscopic and spectroscopic tools complemented with complex impedance measurements. Possible biological applications for in vitro electrophysiological measurements were evaluated based on cell cultures of primary neurons, seeded directly to the chip surface. It was shown that activity can be measured over six months.

KW - Low-temperature cofired ceramics (LTCC)

KW - three-dimensional hybrid multi-electrode array (3D MEA)

KW - in vitro cell culture

KW - thick-film gold electrode

KW - primary neuron culture

KW - GROWTH

U2 - 10.4416/JCST2015-00056

DO - 10.4416/JCST2015-00056

M3 - Article

VL - 6

SP - 315

EP - 324

JO - Journal of Ceramic Science and Technology

JF - Journal of Ceramic Science and Technology

SN - 2190-9385

IS - 4

ER -