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Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from scots pine (Pinus sylvestris L.) seedlings using UPLC–orbitrap–MS

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Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from scots pine (Pinus sylvestris L.) seedlings using UPLC–orbitrap–MS. / Tienaho, Jenni; Karonen, Maarit; Muilu–Mäkelä, Riina; Wähälä, Kristiina; Denegri, Eduardo Leon; Franzén, Robert; Karp, Matti; Santala, Ville; Sarjala, Tytti.

julkaisussa: Molecules, Vuosikerta 24, Nro 12, 2330, 2019.

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Tienaho, Jenni ; Karonen, Maarit ; Muilu–Mäkelä, Riina ; Wähälä, Kristiina ; Denegri, Eduardo Leon ; Franzén, Robert ; Karp, Matti ; Santala, Ville ; Sarjala, Tytti. / Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from scots pine (Pinus sylvestris L.) seedlings using UPLC–orbitrap–MS. Julkaisussa: Molecules. 2019 ; Vuosikerta 24, Nro 12.

Bibtex - Lataa

@article{53496b57b2cb462f92cd99d98588d958,
title = "Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from scots pine (Pinus sylvestris L.) seedlings using UPLC–orbitrap–MS",
abstract = "Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC–DAD–ESI–MS–Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.",
keywords = "Acephala applanata, Coniochaeta mutabilis, Endophytes, Endophytic fungi, Humicolopsis cephalosporioides, Metabolites, Peptides, Phialocephala fortinii, Scots pine, UPLC–MS",
author = "Jenni Tienaho and Maarit Karonen and Riina Muilu–M{\"a}kel{\"a} and Kristiina W{\"a}h{\"a}l{\"a} and Denegri, {Eduardo Leon} and Robert Franz{\'e}n and Matti Karp and Ville Santala and Tytti Sarjala",
note = "EXT={"}Franz{\'e}n, Robert{"}",
year = "2019",
doi = "10.3390/molecules24122330",
language = "English",
volume = "24",
journal = "Molecules",
issn = "1420-3049",
publisher = "MDPI",
number = "12",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from scots pine (Pinus sylvestris L.) seedlings using UPLC–orbitrap–MS

AU - Tienaho, Jenni

AU - Karonen, Maarit

AU - Muilu–Mäkelä, Riina

AU - Wähälä, Kristiina

AU - Denegri, Eduardo Leon

AU - Franzén, Robert

AU - Karp, Matti

AU - Santala, Ville

AU - Sarjala, Tytti

N1 - EXT="Franzén, Robert"

PY - 2019

Y1 - 2019

N2 - Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC–DAD–ESI–MS–Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

AB - Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC–DAD–ESI–MS–Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

KW - Acephala applanata

KW - Coniochaeta mutabilis

KW - Endophytes

KW - Endophytic fungi

KW - Humicolopsis cephalosporioides

KW - Metabolites

KW - Peptides

KW - Phialocephala fortinii

KW - Scots pine

KW - UPLC–MS

U2 - 10.3390/molecules24122330

DO - 10.3390/molecules24122330

M3 - Article

VL - 24

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 12

M1 - 2330

ER -