TUTCRIS - Tampereen teknillinen yliopisto

TUTCRIS

Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
OtsikkoCMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering
ToimittajatAlmir Badnjevic, Lejla Gurbeta Pokvić, Ranko Škrbić, Almir Badnjevic, Lejla Gurbeta Pokvić
KustantajaSpringer Verlag
Sivut21-27
Sivumäärä7
ISBN (painettu)9783030179700
DOI - pysyväislinkit
TilaJulkaistu - 11 toukokuuta 2019
OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisussa
TapahtumaInternational Conference on Medical and Biological Engineering in Bosnia and Herzegovina - Banja Luka, Bosnia-Herzegovina
Kesto: 16 toukokuuta 201918 toukokuuta 2019

Julkaisusarja

NimiIFMBE Proceedings
Vuosikerta73
ISSN (painettu)1680-0737

Conference

ConferenceInternational Conference on Medical and Biological Engineering in Bosnia and Herzegovina
MaaBosnia-Herzegovina
KaupunkiBanja Luka
Ajanjakso16/05/1918/05/19

Tiivistelmä

Cellular mechanobiology is highly important for tissue development and disease formation. However, lack of proper tools limit investigation of the cellular responses to different mechanical cues. High frequency (HF) vibration has already been applied in different cellular applications, but the knowledge of the stimulation effect on cells is limited. To meet this challenge, we designed a HF vibration stimulator for combined mechanical manipulation of live cells and high-resolution light-microscopy. Our system utilizes a commercial miniaturized speaker to vibrate a 3D printed sample vehicle horizontally. Technical tests demonstrated excellent performance at lower frequencies (30–60 Hz), enabling even high magnitude (HMHF, G peak ≥ 1 G peak ) method. Real-time acceleration measurement and light-microscopy both revealed accurately and precisely produced low magnitude (LMHF, G peak < 1 G peak ) vibrations. With our system, we could observe cellular responses to the LMHF (0.2 G peak , 30 Hz) vibration. In this paper, we introduce an inexpensive stimulation platform for the mechanobiology research of different cell applications.

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