TUTCRIS

TY - JOUR

T1 - Mise au point d'un modèle animal original de cicatrice chéloïde

AU - Philandrianos, C.

AU - Gonnelli, D.

AU - Andrac-Meyer, L.

AU - Bruno, M.

AU - Magalon, G.

AU - Mordon, S.

PY - 2014

Y1 - 2014

N2 - Keloid scar is a proliferative healing dysfunction formed by an excessive build-up of collagen fibers on the dermis. It is responsible of aesthetic and functional disabilities. There is no ideal treatment and recurrence occurs very often. Keloid scars occur only to human, that's why animal model needs to be made to study this pathology and new treatments. Few models have been described using human keloid scars implanted into subcutaneous tissue of nude mice or rat. To allow study of topical and laser treatment we have developed a new animal model using human keloid scar fragment with epidermal and dermal tissue implanted into back of nude mice like a full thickness skin graft. Keloid fragments from five donors have been grafted onto 40 nudes mice. Macroscopic and microscopic studies have been made at day 28, 56, 84 and 112. We observed integration of the fragments in all cases. Hyalinized collagen bundles were observed in all implant biopsies confirming the stability of the keloid architecture within 112 days. This model is easily reproducible and allows the study of topical treatment and laser due to the accessibility of the keloid.

AB - Keloid scar is a proliferative healing dysfunction formed by an excessive build-up of collagen fibers on the dermis. It is responsible of aesthetic and functional disabilities. There is no ideal treatment and recurrence occurs very often. Keloid scars occur only to human, that's why animal model needs to be made to study this pathology and new treatments. Few models have been described using human keloid scars implanted into subcutaneous tissue of nude mice or rat. To allow study of topical and laser treatment we have developed a new animal model using human keloid scar fragment with epidermal and dermal tissue implanted into back of nude mice like a full thickness skin graft. Keloid fragments from five donors have been grafted onto 40 nudes mice. Macroscopic and microscopic studies have been made at day 28, 56, 84 and 112. We observed integration of the fragments in all cases. Hyalinized collagen bundles were observed in all implant biopsies confirming the stability of the keloid architecture within 112 days. This model is easily reproducible and allows the study of topical treatment and laser due to the accessibility of the keloid.

KW - Athymic mice

KW - Keloid

KW - Nude mice

KW - Scar

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=84904049452&partnerID=8YFLogxK

U2 - 10.1016/j.anplas.2012.05.001

DO - 10.1016/j.anplas.2012.05.001

M3 - Article

VL - 59

SP - 246

EP - 252

JO - Annales de Chirurgie Plastique Esthétique

JF - Annales de Chirurgie Plastique Esthétique

SN - 0294-1260

IS - 4

ER -