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'miSimBa' - A simulator of synthetic time-lapsed microscopy images of bacterial cells

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Yksityiskohdat

AlkuperäiskieliEnglanti
OtsikkoProceedings - 2015 IEEE 4th Portuguese Meeting on Bioengineering, ENBENG 2015
KustantajaThe Institute of Electrical and Electronics Engineers, Inc.
ISBN (painettu)9781479982691
DOI - pysyväislinkit
TilaJulkaistu - 17 huhtikuuta 2015
OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisussa
TapahtumaPortuguese Meeting on Bioengineering - , Portugali
Kesto: 1 tammikuuta 2000 → …

Conference

ConferencePortuguese Meeting on Bioengineering
MaaPortugali
Ajanjakso1/01/00 → …

Tiivistelmä

Escherichia coli is a model organism for the study of multiple biological processes, including gene expression and cellular aging. Recently, these studies started to rely on temporal single cell imaging. To support these efforts, available automated image analysis methods should be improved. One important step is their validation. Ideally, the 'ground truth' of the images should be known, which is possible only in synthetic images. To simulate artificial images of E. coli cells, we are developing the 'miSimBa' tool (Microscopy Image Simulator of Bacterial Cells). 'miSimBa' simulates images that reproduce the spatial and temporal bacterial organization by modelling realistically cell morphology (shape, size and spatial arrangement), cell growth and division, cell motility and some internal functions and intracellular structures, namely, the nucleoid. This tool also incorporates image acquisition parameters that simulate illumination and the primary sources of noise.

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