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Monitoring pH, temperature and humidity in long-term stem cell culture in CO2 incubator

Tutkimustuotosvertaisarvioitu

Standard

Monitoring pH, temperature and humidity in long-term stem cell culture in CO2 incubator. / Rajan, Dhanesh Kattipparambil; Verho, Jarmo; Kreutzer, Joose; Valimaki, Hannu; Ihalainen, Heimo; Lekkala, Jukka; Patrikoski, Mimmi; Miettinen, Susanna.

2017 IEEE International Symposium on Medical Measurements and Applications (MeMeA). IEEE, 2017. s. 470-474.

Tutkimustuotosvertaisarvioitu

Harvard

Rajan, DK, Verho, J, Kreutzer, J, Valimaki, H, Ihalainen, H, Lekkala, J, Patrikoski, M & Miettinen, S 2017, Monitoring pH, temperature and humidity in long-term stem cell culture in CO2 incubator. julkaisussa 2017 IEEE International Symposium on Medical Measurements and Applications (MeMeA). IEEE, Sivut 470-474, Minnesota, Yhdysvallat, 7/05/19. https://doi.org/10.1109/MeMeA.2017.7985922

APA

Rajan, D. K., Verho, J., Kreutzer, J., Valimaki, H., Ihalainen, H., Lekkala, J., ... Miettinen, S. (2017). Monitoring pH, temperature and humidity in long-term stem cell culture in CO2 incubator. teoksessa 2017 IEEE International Symposium on Medical Measurements and Applications (MeMeA) (Sivut 470-474). IEEE. https://doi.org/10.1109/MeMeA.2017.7985922

Vancouver

Rajan DK, Verho J, Kreutzer J, Valimaki H, Ihalainen H, Lekkala J et al. Monitoring pH, temperature and humidity in long-term stem cell culture in CO2 incubator. julkaisussa 2017 IEEE International Symposium on Medical Measurements and Applications (MeMeA). IEEE. 2017. s. 470-474 https://doi.org/10.1109/MeMeA.2017.7985922

Author

Rajan, Dhanesh Kattipparambil ; Verho, Jarmo ; Kreutzer, Joose ; Valimaki, Hannu ; Ihalainen, Heimo ; Lekkala, Jukka ; Patrikoski, Mimmi ; Miettinen, Susanna. / Monitoring pH, temperature and humidity in long-term stem cell culture in CO2 incubator. 2017 IEEE International Symposium on Medical Measurements and Applications (MeMeA). IEEE, 2017. Sivut 470-474

Bibtex - Lataa

@inproceedings{663ddfbf5faf467993897c3240315033,
title = "Monitoring pH, temperature and humidity in long-term stem cell culture in CO2 incubator",
abstract = "Cell culture in stem cell research is on the rise, not only for basic research but also for its potential medical and therapeutic applications. Monitoring culture process using sensors throughout the culture helps to optimize culture conditions for optimal growth and maximize yield from the cells. Further, the sensor data gives insight into developing better cell culture systems. Typical stem cell culture platforms, e.g. well plates or flasks, cannot be easily equipped with sensors and impose many challenges in periodic process measurements in a CO2 incubator. We present an incubator compatible modular measurement system with three sensors, to monitor pH, temperature and humidity continuously throughout the culture. Sensors are assembled around a flow through cuvette for highly sterile non-contact measurements. No sample preparation or sample extraction from the incubator is needed and the measurements are carried out in a closed flow loop without wasting any medium. The modular assembly is novel, reusable and feasible for humid incubator environments. The system has been tested, validated and used in mesenchymal stem cell expansion and differentiation, for periods ranging from two to three weeks. Once the measurement has commenced at the beginning of culture, continuous measurements without sensor recalibration or special manual attention are carried out till the end of the culture. Measurement data clearly show the interplay between measured parameters, indicating a few stress sources present all through the culture. Additionally, it gives an overall picture of behavior of critical control parameters in an incubator and points out the need for bioprocess systems with automatic process monitoring and smart control for maximum yield, optimal growth and maintenance of the cells.",
keywords = "cell culture incubator environmental monitoring, incubator compatible sensors, long-term cell culture measurements, long-term optical cell culture pH measurements, non-contact cell culture measurements",
author = "Rajan, {Dhanesh Kattipparambil} and Jarmo Verho and Joose Kreutzer and Hannu Valimaki and Heimo Ihalainen and Jukka Lekkala and Mimmi Patrikoski and Susanna Miettinen",
year = "2017",
month = "7",
day = "19",
doi = "10.1109/MeMeA.2017.7985922",
language = "English",
pages = "470--474",
booktitle = "2017 IEEE International Symposium on Medical Measurements and Applications (MeMeA)",
publisher = "IEEE",

}

RIS (suitable for import to EndNote) - Lataa

TY - GEN

T1 - Monitoring pH, temperature and humidity in long-term stem cell culture in CO2 incubator

AU - Rajan, Dhanesh Kattipparambil

AU - Verho, Jarmo

AU - Kreutzer, Joose

AU - Valimaki, Hannu

AU - Ihalainen, Heimo

AU - Lekkala, Jukka

AU - Patrikoski, Mimmi

AU - Miettinen, Susanna

PY - 2017/7/19

Y1 - 2017/7/19

N2 - Cell culture in stem cell research is on the rise, not only for basic research but also for its potential medical and therapeutic applications. Monitoring culture process using sensors throughout the culture helps to optimize culture conditions for optimal growth and maximize yield from the cells. Further, the sensor data gives insight into developing better cell culture systems. Typical stem cell culture platforms, e.g. well plates or flasks, cannot be easily equipped with sensors and impose many challenges in periodic process measurements in a CO2 incubator. We present an incubator compatible modular measurement system with three sensors, to monitor pH, temperature and humidity continuously throughout the culture. Sensors are assembled around a flow through cuvette for highly sterile non-contact measurements. No sample preparation or sample extraction from the incubator is needed and the measurements are carried out in a closed flow loop without wasting any medium. The modular assembly is novel, reusable and feasible for humid incubator environments. The system has been tested, validated and used in mesenchymal stem cell expansion and differentiation, for periods ranging from two to three weeks. Once the measurement has commenced at the beginning of culture, continuous measurements without sensor recalibration or special manual attention are carried out till the end of the culture. Measurement data clearly show the interplay between measured parameters, indicating a few stress sources present all through the culture. Additionally, it gives an overall picture of behavior of critical control parameters in an incubator and points out the need for bioprocess systems with automatic process monitoring and smart control for maximum yield, optimal growth and maintenance of the cells.

AB - Cell culture in stem cell research is on the rise, not only for basic research but also for its potential medical and therapeutic applications. Monitoring culture process using sensors throughout the culture helps to optimize culture conditions for optimal growth and maximize yield from the cells. Further, the sensor data gives insight into developing better cell culture systems. Typical stem cell culture platforms, e.g. well plates or flasks, cannot be easily equipped with sensors and impose many challenges in periodic process measurements in a CO2 incubator. We present an incubator compatible modular measurement system with three sensors, to monitor pH, temperature and humidity continuously throughout the culture. Sensors are assembled around a flow through cuvette for highly sterile non-contact measurements. No sample preparation or sample extraction from the incubator is needed and the measurements are carried out in a closed flow loop without wasting any medium. The modular assembly is novel, reusable and feasible for humid incubator environments. The system has been tested, validated and used in mesenchymal stem cell expansion and differentiation, for periods ranging from two to three weeks. Once the measurement has commenced at the beginning of culture, continuous measurements without sensor recalibration or special manual attention are carried out till the end of the culture. Measurement data clearly show the interplay between measured parameters, indicating a few stress sources present all through the culture. Additionally, it gives an overall picture of behavior of critical control parameters in an incubator and points out the need for bioprocess systems with automatic process monitoring and smart control for maximum yield, optimal growth and maintenance of the cells.

KW - cell culture incubator environmental monitoring

KW - incubator compatible sensors

KW - long-term cell culture measurements

KW - long-term optical cell culture pH measurements

KW - non-contact cell culture measurements

U2 - 10.1109/MeMeA.2017.7985922

DO - 10.1109/MeMeA.2017.7985922

M3 - Conference contribution

SP - 470

EP - 474

BT - 2017 IEEE International Symposium on Medical Measurements and Applications (MeMeA)

PB - IEEE

ER -