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Porous poly-L-lactide-co-1-caprolactone scaffold: A novel biomaterial for vaginal tissue engineering

Tutkimustuotosvertaisarvioitu

Standard

Porous poly-L-lactide-co-1-caprolactone scaffold : A novel biomaterial for vaginal tissue engineering. / Sartoneva, Reetta; Kuismanen, Kirsi; Juntunen, Miia; Karjalainen, Sanna; Hannula, Markus; Kyllönen, Laura; Hyttinen, Jari; Huhtala, Heini; Paakinaho, Kaarlo; Miettinen, Susanna.

julkaisussa: Royal Society Open Science, Vuosikerta 5, Nro 8, 180811, 01.08.2018.

Tutkimustuotosvertaisarvioitu

Harvard

Sartoneva, R, Kuismanen, K, Juntunen, M, Karjalainen, S, Hannula, M, Kyllönen, L, Hyttinen, J, Huhtala, H, Paakinaho, K & Miettinen, S 2018, 'Porous poly-L-lactide-co-1-caprolactone scaffold: A novel biomaterial for vaginal tissue engineering', Royal Society Open Science, Vuosikerta. 5, Nro 8, 180811. https://doi.org/10.1098/rsos.180811

APA

Sartoneva, R., Kuismanen, K., Juntunen, M., Karjalainen, S., Hannula, M., Kyllönen, L., ... Miettinen, S. (2018). Porous poly-L-lactide-co-1-caprolactone scaffold: A novel biomaterial for vaginal tissue engineering. Royal Society Open Science, 5(8), [180811]. https://doi.org/10.1098/rsos.180811

Vancouver

Sartoneva R, Kuismanen K, Juntunen M, Karjalainen S, Hannula M, Kyllönen L et al. Porous poly-L-lactide-co-1-caprolactone scaffold: A novel biomaterial for vaginal tissue engineering. Royal Society Open Science. 2018 elo 1;5(8). 180811. https://doi.org/10.1098/rsos.180811

Author

Sartoneva, Reetta ; Kuismanen, Kirsi ; Juntunen, Miia ; Karjalainen, Sanna ; Hannula, Markus ; Kyllönen, Laura ; Hyttinen, Jari ; Huhtala, Heini ; Paakinaho, Kaarlo ; Miettinen, Susanna. / Porous poly-L-lactide-co-1-caprolactone scaffold : A novel biomaterial for vaginal tissue engineering. Julkaisussa: Royal Society Open Science. 2018 ; Vuosikerta 5, Nro 8.

Bibtex - Lataa

@article{04b675d63f8f4046aa1380f45a4ada1a,
title = "Porous poly-L-lactide-co-1-caprolactone scaffold: A novel biomaterial for vaginal tissue engineering",
abstract = "The surgical reconstruction of functional neovagina is challenging and susceptible to complications. Therefore, developing tissue engineering-based treatment methods for vaginal defects is important. Our aim was to develop and test a novel supercritical carbon dioxide foamed poly-L-lactide-co-1-caprolactone (scPLCL) scaffold for vaginal reconstruction. The scaffolds were manufactured and characterized for porosity (65 + 4{\%}), pore size (350 + 150 mm) and elastic modulus (2.8 + 0.4 MPa). Vaginal epithelial (EC) and stromal cells (SC) were isolated, expanded and characterized with flow cytometry. Finally, cells were cultured with scPLCL scaffolds in separate and/or co-cultures. Their attachment, viability, proliferation and phenotype were analysed. Both cell types strongly expressed cell surface markers CD44, CD73 and CD166. Strong expression of CD326 was detected with ECs and CD90 and CD105 with SCs. Both ECs and SCs attached and maintained viability on scPLCL. Further, scPLCL supported the proliferation of especially ECs, which also maintained epithelial phenotype (cytokeratin expression) during 14-day assessment period. Interestingly, ECs expressed uroplakin (UP) Ia, UPIb and UPIII markers; further, UPIa and UPIII expression was significantly higher on ECs cultured on scPLCL than on cell culture plastic.",
keywords = "Cell characterization, Neovagina, Poly-L-lactide-co-1-caprolactone, Vaginal epithelial cell, Vaginal stromal cell, Vaginal tissue engineering",
author = "Reetta Sartoneva and Kirsi Kuismanen and Miia Juntunen and Sanna Karjalainen and Markus Hannula and Laura Kyll{\"o}nen and Jari Hyttinen and Heini Huhtala and Kaarlo Paakinaho and Susanna Miettinen",
year = "2018",
month = "8",
day = "1",
doi = "10.1098/rsos.180811",
language = "English",
volume = "5",
journal = "Royal Society Open Science",
issn = "2054-5703",
publisher = "Royal Society, The",
number = "8",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Porous poly-L-lactide-co-1-caprolactone scaffold

T2 - A novel biomaterial for vaginal tissue engineering

AU - Sartoneva, Reetta

AU - Kuismanen, Kirsi

AU - Juntunen, Miia

AU - Karjalainen, Sanna

AU - Hannula, Markus

AU - Kyllönen, Laura

AU - Hyttinen, Jari

AU - Huhtala, Heini

AU - Paakinaho, Kaarlo

AU - Miettinen, Susanna

PY - 2018/8/1

Y1 - 2018/8/1

N2 - The surgical reconstruction of functional neovagina is challenging and susceptible to complications. Therefore, developing tissue engineering-based treatment methods for vaginal defects is important. Our aim was to develop and test a novel supercritical carbon dioxide foamed poly-L-lactide-co-1-caprolactone (scPLCL) scaffold for vaginal reconstruction. The scaffolds were manufactured and characterized for porosity (65 + 4%), pore size (350 + 150 mm) and elastic modulus (2.8 + 0.4 MPa). Vaginal epithelial (EC) and stromal cells (SC) were isolated, expanded and characterized with flow cytometry. Finally, cells were cultured with scPLCL scaffolds in separate and/or co-cultures. Their attachment, viability, proliferation and phenotype were analysed. Both cell types strongly expressed cell surface markers CD44, CD73 and CD166. Strong expression of CD326 was detected with ECs and CD90 and CD105 with SCs. Both ECs and SCs attached and maintained viability on scPLCL. Further, scPLCL supported the proliferation of especially ECs, which also maintained epithelial phenotype (cytokeratin expression) during 14-day assessment period. Interestingly, ECs expressed uroplakin (UP) Ia, UPIb and UPIII markers; further, UPIa and UPIII expression was significantly higher on ECs cultured on scPLCL than on cell culture plastic.

AB - The surgical reconstruction of functional neovagina is challenging and susceptible to complications. Therefore, developing tissue engineering-based treatment methods for vaginal defects is important. Our aim was to develop and test a novel supercritical carbon dioxide foamed poly-L-lactide-co-1-caprolactone (scPLCL) scaffold for vaginal reconstruction. The scaffolds were manufactured and characterized for porosity (65 + 4%), pore size (350 + 150 mm) and elastic modulus (2.8 + 0.4 MPa). Vaginal epithelial (EC) and stromal cells (SC) were isolated, expanded and characterized with flow cytometry. Finally, cells were cultured with scPLCL scaffolds in separate and/or co-cultures. Their attachment, viability, proliferation and phenotype were analysed. Both cell types strongly expressed cell surface markers CD44, CD73 and CD166. Strong expression of CD326 was detected with ECs and CD90 and CD105 with SCs. Both ECs and SCs attached and maintained viability on scPLCL. Further, scPLCL supported the proliferation of especially ECs, which also maintained epithelial phenotype (cytokeratin expression) during 14-day assessment period. Interestingly, ECs expressed uroplakin (UP) Ia, UPIb and UPIII markers; further, UPIa and UPIII expression was significantly higher on ECs cultured on scPLCL than on cell culture plastic.

KW - Cell characterization

KW - Neovagina

KW - Poly-L-lactide-co-1-caprolactone

KW - Vaginal epithelial cell

KW - Vaginal stromal cell

KW - Vaginal tissue engineering

U2 - 10.1098/rsos.180811

DO - 10.1098/rsos.180811

M3 - Article

VL - 5

JO - Royal Society Open Science

JF - Royal Society Open Science

SN - 2054-5703

IS - 8

M1 - 180811

ER -