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Rapid and sensitive colorimetric ELISA using silver nanoparticles, microwaves and split ring resonator structures

Tutkimustuotosvertaisarvioitu

Standard

Rapid and sensitive colorimetric ELISA using silver nanoparticles, microwaves and split ring resonator structures. / Addae, Sarah A.; Pinard, Melissa A.; Caglayan, Humeyra; Cakmakyapan, Semih; Caliskan, Deniz; Ozbay, Ekmel; Aslan, Kadir.

julkaisussa: Nano Biomedicine and Engineering, Vuosikerta 2, Nro 3, 2010, s. 155-164.

Tutkimustuotosvertaisarvioitu

Harvard

Addae, SA, Pinard, MA, Caglayan, H, Cakmakyapan, S, Caliskan, D, Ozbay, E & Aslan, K 2010, 'Rapid and sensitive colorimetric ELISA using silver nanoparticles, microwaves and split ring resonator structures', Nano Biomedicine and Engineering, Vuosikerta. 2, Nro 3, Sivut 155-164. https://doi.org/10.5101/nbe.v2i3.p155-164

APA

Addae, S. A., Pinard, M. A., Caglayan, H., Cakmakyapan, S., Caliskan, D., Ozbay, E., & Aslan, K. (2010). Rapid and sensitive colorimetric ELISA using silver nanoparticles, microwaves and split ring resonator structures. Nano Biomedicine and Engineering, 2(3), 155-164. https://doi.org/10.5101/nbe.v2i3.p155-164

Vancouver

Addae SA, Pinard MA, Caglayan H, Cakmakyapan S, Caliskan D, Ozbay E et al. Rapid and sensitive colorimetric ELISA using silver nanoparticles, microwaves and split ring resonator structures. Nano Biomedicine and Engineering. 2010;2(3):155-164. https://doi.org/10.5101/nbe.v2i3.p155-164

Author

Addae, Sarah A. ; Pinard, Melissa A. ; Caglayan, Humeyra ; Cakmakyapan, Semih ; Caliskan, Deniz ; Ozbay, Ekmel ; Aslan, Kadir. / Rapid and sensitive colorimetric ELISA using silver nanoparticles, microwaves and split ring resonator structures. Julkaisussa: Nano Biomedicine and Engineering. 2010 ; Vuosikerta 2, Nro 3. Sivut 155-164.

Bibtex - Lataa

@article{ba1c9f2526fa42138ce1da736adadd20,
title = "Rapid and sensitive colorimetric ELISA using silver nanoparticles, microwaves and split ring resonator structures",
abstract = "We report a new approach to colorimetric Enzyme-Linked Immunosorbent Assay (ELISA) that reduces the total assay time to < 2 min and the lower-detection-limit by 100-fold based on absorbance readout. The new approach combines the use of silver nanoparticles, microwaves and split ring resonators (SRR). The SRR structure is comprised of a square frame of copper thin film (30 μm thick, 1 mm wide, overall length of ~9.4 mm on each side) with a single split on one side, which was deposited onto a circuit board (2×2 cm2). A single micro-cuvette (10 μl volume capacity) was placed in the split of the SRR structures. Theoretical simulations predict that electric fields are focused in and above the micro-cuvette without the accumulation of electrical charge that breaks down the copper film. Subsequently, the walls and the bottom of the micro-cuvette were coated with silver nanoparticles using a modified Tollen's reaction scheme. The silver nanoparticles served as a mediator for the creation of thermal gradient between the bioassay medium and the silver surface, where the bioassay is constructed. Upon exposure to low power microwave heating, the bioassay medium in the micro-cuvette was rapidly and uniformly heated by the focused electric fields. In addition, the creation of thermal gradient resulted in the rapid assembly of the proteins on the surface of silver nanoparticles without denaturing the proteins. The proof-of-principle of the new approach to ELISA was demonstrated for the detection of a model protein (biotinylated-bovine serum albumin, b-BSA). In this regard, the detection of b-BSA with bulk concentrations (1 μM to 1 pM) was carried out on commercially available 96-well high throughput screening (HTS) plates and silver nanoparticle-deposited SRR structures at room temperature and with microwave heating, respectively. While the room temperature bioassay (without microwave heating) took 70 min to complete, the identical bioassay took < 2 min to complete using the SRR structures (with microwave heating). A lower detection limit of 0.01 nM for b-BSA (100-fold lower than room temperature ELISA) was observed using the SRR structures.",
keywords = "Bioassays, ELISA, Immunoassays, Silver colloids, Silver island films",
author = "Addae, {Sarah A.} and Pinard, {Melissa A.} and Humeyra Caglayan and Semih Cakmakyapan and Deniz Caliskan and Ekmel Ozbay and Kadir Aslan",
note = "EXT={"}Caglayan, Humeyra{"}",
year = "2010",
doi = "10.5101/nbe.v2i3.p155-164",
language = "English",
volume = "2",
pages = "155--164",
journal = "Nano Biomedicine and Engineering",
issn = "2150-5578",
publisher = "Open Access House of Science and Technology (OAHOST)",
number = "3",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Rapid and sensitive colorimetric ELISA using silver nanoparticles, microwaves and split ring resonator structures

AU - Addae, Sarah A.

AU - Pinard, Melissa A.

AU - Caglayan, Humeyra

AU - Cakmakyapan, Semih

AU - Caliskan, Deniz

AU - Ozbay, Ekmel

AU - Aslan, Kadir

N1 - EXT="Caglayan, Humeyra"

PY - 2010

Y1 - 2010

N2 - We report a new approach to colorimetric Enzyme-Linked Immunosorbent Assay (ELISA) that reduces the total assay time to < 2 min and the lower-detection-limit by 100-fold based on absorbance readout. The new approach combines the use of silver nanoparticles, microwaves and split ring resonators (SRR). The SRR structure is comprised of a square frame of copper thin film (30 μm thick, 1 mm wide, overall length of ~9.4 mm on each side) with a single split on one side, which was deposited onto a circuit board (2×2 cm2). A single micro-cuvette (10 μl volume capacity) was placed in the split of the SRR structures. Theoretical simulations predict that electric fields are focused in and above the micro-cuvette without the accumulation of electrical charge that breaks down the copper film. Subsequently, the walls and the bottom of the micro-cuvette were coated with silver nanoparticles using a modified Tollen's reaction scheme. The silver nanoparticles served as a mediator for the creation of thermal gradient between the bioassay medium and the silver surface, where the bioassay is constructed. Upon exposure to low power microwave heating, the bioassay medium in the micro-cuvette was rapidly and uniformly heated by the focused electric fields. In addition, the creation of thermal gradient resulted in the rapid assembly of the proteins on the surface of silver nanoparticles without denaturing the proteins. The proof-of-principle of the new approach to ELISA was demonstrated for the detection of a model protein (biotinylated-bovine serum albumin, b-BSA). In this regard, the detection of b-BSA with bulk concentrations (1 μM to 1 pM) was carried out on commercially available 96-well high throughput screening (HTS) plates and silver nanoparticle-deposited SRR structures at room temperature and with microwave heating, respectively. While the room temperature bioassay (without microwave heating) took 70 min to complete, the identical bioassay took < 2 min to complete using the SRR structures (with microwave heating). A lower detection limit of 0.01 nM for b-BSA (100-fold lower than room temperature ELISA) was observed using the SRR structures.

AB - We report a new approach to colorimetric Enzyme-Linked Immunosorbent Assay (ELISA) that reduces the total assay time to < 2 min and the lower-detection-limit by 100-fold based on absorbance readout. The new approach combines the use of silver nanoparticles, microwaves and split ring resonators (SRR). The SRR structure is comprised of a square frame of copper thin film (30 μm thick, 1 mm wide, overall length of ~9.4 mm on each side) with a single split on one side, which was deposited onto a circuit board (2×2 cm2). A single micro-cuvette (10 μl volume capacity) was placed in the split of the SRR structures. Theoretical simulations predict that electric fields are focused in and above the micro-cuvette without the accumulation of electrical charge that breaks down the copper film. Subsequently, the walls and the bottom of the micro-cuvette were coated with silver nanoparticles using a modified Tollen's reaction scheme. The silver nanoparticles served as a mediator for the creation of thermal gradient between the bioassay medium and the silver surface, where the bioassay is constructed. Upon exposure to low power microwave heating, the bioassay medium in the micro-cuvette was rapidly and uniformly heated by the focused electric fields. In addition, the creation of thermal gradient resulted in the rapid assembly of the proteins on the surface of silver nanoparticles without denaturing the proteins. The proof-of-principle of the new approach to ELISA was demonstrated for the detection of a model protein (biotinylated-bovine serum albumin, b-BSA). In this regard, the detection of b-BSA with bulk concentrations (1 μM to 1 pM) was carried out on commercially available 96-well high throughput screening (HTS) plates and silver nanoparticle-deposited SRR structures at room temperature and with microwave heating, respectively. While the room temperature bioassay (without microwave heating) took 70 min to complete, the identical bioassay took < 2 min to complete using the SRR structures (with microwave heating). A lower detection limit of 0.01 nM for b-BSA (100-fold lower than room temperature ELISA) was observed using the SRR structures.

KW - Bioassays

KW - ELISA

KW - Immunoassays

KW - Silver colloids

KW - Silver island films

UR - http://www.scopus.com/inward/record.url?scp=80053225008&partnerID=8YFLogxK

U2 - 10.5101/nbe.v2i3.p155-164

DO - 10.5101/nbe.v2i3.p155-164

M3 - Article

VL - 2

SP - 155

EP - 164

JO - Nano Biomedicine and Engineering

JF - Nano Biomedicine and Engineering

SN - 2150-5578

IS - 3

ER -