TUTCRIS - Tampereen teknillinen yliopisto

TUTCRIS

Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut507-514
Sivumäärä8
JulkaisuSensors and Actuators B: Chemical
Vuosikerta221
DOI - pysyväislinkit
TilaJulkaistu - 31 joulukuuta 2015
OKM-julkaisutyyppiA1 Alkuperäisartikkeli

Tiivistelmä

Here, we describe the use of a biolayer interferometry biosensor for the fast and sensitive detection of virus-specific antibodies from human serum samples. Norovirus-like particles and norovirus P-particles were used to functionalise the biosensor tip. The detection of antibodies directly from serum samples was challenging, but the addition of a metal chelator (DAB) combined with an anti-human horseradish peroxidase-tagged antibody enabled enhanced detection of virus-specific antibodies in serum dilutions up to 1:100,000. Biolayer interferometry provides results faster than an ELISA, with results in as little as 10-20 min when using pre-functionalised sensors. Therefore, biolayer interferometry combined with DAB enhancement offers an attractive method for quick and sensitive quantification of biomolecules from complicated sample matrices.