Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor
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Yksityiskohdat
| Alkuperäiskieli | Englanti |
|---|---|
| Sivut | 507-514 |
| Sivumäärä | 8 |
| Julkaisu | Sensors and Actuators B: Chemical |
| Vuosikerta | 221 |
| DOI - pysyväislinkit | |
| Tila | Julkaistu - 31 joulukuuta 2015 |
| OKM-julkaisutyyppi | A1 Alkuperäisartikkeli |
Tiivistelmä
Here, we describe the use of a biolayer interferometry biosensor for the fast and sensitive detection of virus-specific antibodies from human serum samples. Norovirus-like particles and norovirus P-particles were used to functionalise the biosensor tip. The detection of antibodies directly from serum samples was challenging, but the addition of a metal chelator (DAB) combined with an anti-human horseradish peroxidase-tagged antibody enabled enhanced detection of virus-specific antibodies in serum dilutions up to 1:100,000. Biolayer interferometry provides results faster than an ELISA, with results in as little as 10-20 min when using pre-functionalised sensors. Therefore, biolayer interferometry combined with DAB enhancement offers an attractive method for quick and sensitive quantification of biomolecules from complicated sample matrices.