TUTCRIS - Tampereen teknillinen yliopisto

TUTCRIS

Redox-coupled proton transfer in the respiratory complex I

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
Tuotoksen mediaPoster
JulkaisijaEuropean Biophysics Journal
Vuosikerta44
TilaJulkaistu - 2015

Tiivistelmä

Complex I functions as a redox-driven proton pump in the
aerobic respiratory chains of bacteria and eukaryotes. Re-
markably, the electron transfer domain of complex I is sepa-
rated by
ca
. 200
̊
A from the most distant proton pathways.
To understand the molecular mechanism of this biomolecular
machine, we perform here large-scale classical and quantum
molecular simulations, and site-directed mutagenesis experi-
ments, to study how the quinone reduction triggers the func-
tion of the proton pump. We find that coupled electrostatic,
conformational, and hydration changes are central for estab-
lishing the long-range energy coupling in complex I.