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TUTCRIS

Reproducible preparation method of hydrogels for cell culture applications – Case study with spermidine crosslinked gellan gum

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
OtsikkoEMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017
KustantajaSpringer Verlag
Sivut811-814
Sivumäärä4
ISBN (painettu)9789811051210
DOI - pysyväislinkit
TilaJulkaistu - 2018
OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisussa
TapahtumaJoint Conference of the European Medical and Biological Engineering Conference (EMBEC) and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC) -
Kesto: 1 tammikuuta 1900 → …

Julkaisusarja

NimiIFMBE Proceedings
Vuosikerta65
ISSN (painettu)1680-0737

Conference

ConferenceJoint Conference of the European Medical and Biological Engineering Conference (EMBEC) and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC)
Ajanjakso1/01/00 → …

Tiivistelmä

Hydrogels are promising materials to culture cells in 3D environment. Their mechanical properties are decisive, as cells understand the stiffness of their surroundings. Herein, a method is presented to produce ionically crosslinked hydrogel matrices. A reproducible method is needed, because conventional methods cause inconsistent properties. The investigated material is gellan gum, crosslinked with the bioamine spermidine. Samples were prepared with the more conventional ‘pipetting’ technique and with self-developed ‘uniform mixing’ technique. The two preparation techniques are described in detail and the obtained hydrogels are compared. The mechanical properties are analyzed with compression testing. The obtained results show that samples by the so-called ‘uniform mixing’ method have more uniform dimensions and higher compression modulus. A preliminary stability test in cell culture medium was also carried out.

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