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Rewiring the wax ester production pathway of acinetobacter baylyi ADP1

Tutkimustuotosvertaisarvioitu

Standard

Rewiring the wax ester production pathway of acinetobacter baylyi ADP1. / Santala, Suvi; Efimova, Elena; Koskinen, Perttu; Karp, Matti Tapani; Santala, Ville.

julkaisussa: ACS Synthetic Biology, Vuosikerta 3, Nro 3, 21.03.2014, s. 145-151.

Tutkimustuotosvertaisarvioitu

Harvard

Santala, S, Efimova, E, Koskinen, P, Karp, MT & Santala, V 2014, 'Rewiring the wax ester production pathway of acinetobacter baylyi ADP1', ACS Synthetic Biology, Vuosikerta. 3, Nro 3, Sivut 145-151. https://doi.org/10.1021/sb4000788

APA

Santala, S., Efimova, E., Koskinen, P., Karp, M. T., & Santala, V. (2014). Rewiring the wax ester production pathway of acinetobacter baylyi ADP1. ACS Synthetic Biology, 3(3), 145-151. https://doi.org/10.1021/sb4000788

Vancouver

Santala S, Efimova E, Koskinen P, Karp MT, Santala V. Rewiring the wax ester production pathway of acinetobacter baylyi ADP1. ACS Synthetic Biology. 2014 maalis 21;3(3):145-151. https://doi.org/10.1021/sb4000788

Author

Santala, Suvi ; Efimova, Elena ; Koskinen, Perttu ; Karp, Matti Tapani ; Santala, Ville. / Rewiring the wax ester production pathway of acinetobacter baylyi ADP1. Julkaisussa: ACS Synthetic Biology. 2014 ; Vuosikerta 3, Nro 3. Sivut 145-151.

Bibtex - Lataa

@article{9f0f7eeb699f48aab60f3fb274a0c38e,
title = "Rewiring the wax ester production pathway of acinetobacter baylyi ADP1",
abstract = "Wax esters are industrially relevant high-value molecules. For sustainable production of wax esters, bacterial cell factories are suggested to replace the chemical processes exploiting expensive starting materials. However, it is well recognized that new sophisticated solutions employing synthetic biology toolbox are required to improve and tune the cellular production platform to meet the product requirements. For example, saturated wax esters with alkanol chain lengths C12 or C14 that are convenient for industrial uses are rare among bacteria. Acinetobacter baylyi ADP1, a natural producer of wax esters, is a convenient model organism for studying the potentiality and modifiability of wax esters in a natural host by means of synthetic biology. In order to establish a controllable production platform exploiting well-characterized biocomponents, and to modify the wax ester synthesis pathway of A. baylyi ADP1 in terms product quality, a fatty acid reductase complex LuxCDE with an inducible arabinose promoter was employed to replace the natural fatty acyl-CoA reductase acr1 in ADP1. The engineered strain was able to produce wax esters by the introduced synthetic pathway. Moreover, the fatty alkanol chain length profile of wax esters was found to shift toward shorter and more saturated carbon chains, C16:0 accounting for most of the alkanols. The study demonstrates the potentiality of recircuiting a biosynthesis pathway in a natural producer, enabling a regulated production of a customized bioproduct. Furthermore, the LuxCDE complex can be potentially used as a well-characterized biopart in a variety of synthetic biology applications involving the production of long-chain hydrocarbons. {\circledC} 2014 American Chemical Society.",
keywords = "Acinetobacter baylyi ADP1, fatty-acyl CoA reductase, long chain aldehyde, luxCDE, recircuiting, wax ester",
author = "Suvi Santala and Elena Efimova and Perttu Koskinen and Karp, {Matti Tapani} and Ville Santala",
note = "Contribution: organisation=keb,FACT1=1<br/>Portfolio EDEND: 2014-02-15<br/>Publisher name: American Chemical Society",
year = "2014",
month = "3",
day = "21",
doi = "10.1021/sb4000788",
language = "English",
volume = "3",
pages = "145--151",
journal = "ACS Synthetic Biology",
issn = "2161-5063",
publisher = "American Chemical Society",
number = "3",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Rewiring the wax ester production pathway of acinetobacter baylyi ADP1

AU - Santala, Suvi

AU - Efimova, Elena

AU - Koskinen, Perttu

AU - Karp, Matti Tapani

AU - Santala, Ville

N1 - Contribution: organisation=keb,FACT1=1<br/>Portfolio EDEND: 2014-02-15<br/>Publisher name: American Chemical Society

PY - 2014/3/21

Y1 - 2014/3/21

N2 - Wax esters are industrially relevant high-value molecules. For sustainable production of wax esters, bacterial cell factories are suggested to replace the chemical processes exploiting expensive starting materials. However, it is well recognized that new sophisticated solutions employing synthetic biology toolbox are required to improve and tune the cellular production platform to meet the product requirements. For example, saturated wax esters with alkanol chain lengths C12 or C14 that are convenient for industrial uses are rare among bacteria. Acinetobacter baylyi ADP1, a natural producer of wax esters, is a convenient model organism for studying the potentiality and modifiability of wax esters in a natural host by means of synthetic biology. In order to establish a controllable production platform exploiting well-characterized biocomponents, and to modify the wax ester synthesis pathway of A. baylyi ADP1 in terms product quality, a fatty acid reductase complex LuxCDE with an inducible arabinose promoter was employed to replace the natural fatty acyl-CoA reductase acr1 in ADP1. The engineered strain was able to produce wax esters by the introduced synthetic pathway. Moreover, the fatty alkanol chain length profile of wax esters was found to shift toward shorter and more saturated carbon chains, C16:0 accounting for most of the alkanols. The study demonstrates the potentiality of recircuiting a biosynthesis pathway in a natural producer, enabling a regulated production of a customized bioproduct. Furthermore, the LuxCDE complex can be potentially used as a well-characterized biopart in a variety of synthetic biology applications involving the production of long-chain hydrocarbons. © 2014 American Chemical Society.

AB - Wax esters are industrially relevant high-value molecules. For sustainable production of wax esters, bacterial cell factories are suggested to replace the chemical processes exploiting expensive starting materials. However, it is well recognized that new sophisticated solutions employing synthetic biology toolbox are required to improve and tune the cellular production platform to meet the product requirements. For example, saturated wax esters with alkanol chain lengths C12 or C14 that are convenient for industrial uses are rare among bacteria. Acinetobacter baylyi ADP1, a natural producer of wax esters, is a convenient model organism for studying the potentiality and modifiability of wax esters in a natural host by means of synthetic biology. In order to establish a controllable production platform exploiting well-characterized biocomponents, and to modify the wax ester synthesis pathway of A. baylyi ADP1 in terms product quality, a fatty acid reductase complex LuxCDE with an inducible arabinose promoter was employed to replace the natural fatty acyl-CoA reductase acr1 in ADP1. The engineered strain was able to produce wax esters by the introduced synthetic pathway. Moreover, the fatty alkanol chain length profile of wax esters was found to shift toward shorter and more saturated carbon chains, C16:0 accounting for most of the alkanols. The study demonstrates the potentiality of recircuiting a biosynthesis pathway in a natural producer, enabling a regulated production of a customized bioproduct. Furthermore, the LuxCDE complex can be potentially used as a well-characterized biopart in a variety of synthetic biology applications involving the production of long-chain hydrocarbons. © 2014 American Chemical Society.

KW - Acinetobacter baylyi ADP1

KW - fatty-acyl CoA reductase

KW - long chain aldehyde

KW - luxCDE

KW - recircuiting

KW - wax ester

UR - http://www.scopus.com/inward/record.url?scp=84896925324&partnerID=8YFLogxK

U2 - 10.1021/sb4000788

DO - 10.1021/sb4000788

M3 - Article

VL - 3

SP - 145

EP - 151

JO - ACS Synthetic Biology

JF - ACS Synthetic Biology

SN - 2161-5063

IS - 3

ER -