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Saline Accelerates Oxime Reaction with Aldehyde and Keto Substrates at Physiological pH

Tutkimustuotosvertaisarvioitu

Standard

Saline Accelerates Oxime Reaction with Aldehyde and Keto Substrates at Physiological pH. / Wang, Shujiang; Nawale, Ganesh N.; Kadekar, Sandeep; Podiyan Oommen, Oommen; Jena, Naresh K.; Chakraborty, Sudip; Hilborn, Jöns; Varghese, Oommen P.

julkaisussa: Scientific Reports, Vuosikerta 8, Nro 1, 2193, 01.02.2018.

Tutkimustuotosvertaisarvioitu

Harvard

Wang, S, Nawale, GN, Kadekar, S, Podiyan Oommen, O, Jena, NK, Chakraborty, S, Hilborn, J & Varghese, OP 2018, 'Saline Accelerates Oxime Reaction with Aldehyde and Keto Substrates at Physiological pH', Scientific Reports, Vuosikerta. 8, Nro 1, 2193. https://doi.org/10.1038/s41598-018-20735-0

APA

Wang, S., Nawale, G. N., Kadekar, S., Podiyan Oommen, O., Jena, N. K., Chakraborty, S., ... Varghese, O. P. (2018). Saline Accelerates Oxime Reaction with Aldehyde and Keto Substrates at Physiological pH. Scientific Reports, 8(1), [2193]. https://doi.org/10.1038/s41598-018-20735-0

Vancouver

Wang S, Nawale GN, Kadekar S, Podiyan Oommen O, Jena NK, Chakraborty S et al. Saline Accelerates Oxime Reaction with Aldehyde and Keto Substrates at Physiological pH. Scientific Reports. 2018 helmi 1;8(1). 2193. https://doi.org/10.1038/s41598-018-20735-0

Author

Wang, Shujiang ; Nawale, Ganesh N. ; Kadekar, Sandeep ; Podiyan Oommen, Oommen ; Jena, Naresh K. ; Chakraborty, Sudip ; Hilborn, Jöns ; Varghese, Oommen P. / Saline Accelerates Oxime Reaction with Aldehyde and Keto Substrates at Physiological pH. Julkaisussa: Scientific Reports. 2018 ; Vuosikerta 8, Nro 1.

Bibtex - Lataa

@article{090c8f2df7de44ebaf75684caa04b0db,
title = "Saline Accelerates Oxime Reaction with Aldehyde and Keto Substrates at Physiological pH",
abstract = "We have discovered a simple and versatile reaction condition for oxime mediated bioconjugation reaction that could be adapted for both aldehyde and keto substrates. We found that saline accelerated the oxime kinetics in a concentration-dependent manner under physiological conditions. The reaction mechanism is validated by computational studies, and the versatility of the reaction is demonstrated by cell-surface labeling experiments. Saline offers an efficient and non-toxic catalytic option for performing the bioorthogonal-coupling reaction of biomolecules at the physiological pH. This saline mediated bioconjugation reaction represents the most biofriendly, mild and versatile approach for conjugating sensitive biomolecules and does not require any extensive purification step.",
author = "Shujiang Wang and Nawale, {Ganesh N.} and Sandeep Kadekar and {Podiyan Oommen}, Oommen and Jena, {Naresh K.} and Sudip Chakraborty and J{\"o}ns Hilborn and Varghese, {Oommen P.}",
year = "2018",
month = "2",
day = "1",
doi = "10.1038/s41598-018-20735-0",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Saline Accelerates Oxime Reaction with Aldehyde and Keto Substrates at Physiological pH

AU - Wang, Shujiang

AU - Nawale, Ganesh N.

AU - Kadekar, Sandeep

AU - Podiyan Oommen, Oommen

AU - Jena, Naresh K.

AU - Chakraborty, Sudip

AU - Hilborn, Jöns

AU - Varghese, Oommen P.

PY - 2018/2/1

Y1 - 2018/2/1

N2 - We have discovered a simple and versatile reaction condition for oxime mediated bioconjugation reaction that could be adapted for both aldehyde and keto substrates. We found that saline accelerated the oxime kinetics in a concentration-dependent manner under physiological conditions. The reaction mechanism is validated by computational studies, and the versatility of the reaction is demonstrated by cell-surface labeling experiments. Saline offers an efficient and non-toxic catalytic option for performing the bioorthogonal-coupling reaction of biomolecules at the physiological pH. This saline mediated bioconjugation reaction represents the most biofriendly, mild and versatile approach for conjugating sensitive biomolecules and does not require any extensive purification step.

AB - We have discovered a simple and versatile reaction condition for oxime mediated bioconjugation reaction that could be adapted for both aldehyde and keto substrates. We found that saline accelerated the oxime kinetics in a concentration-dependent manner under physiological conditions. The reaction mechanism is validated by computational studies, and the versatility of the reaction is demonstrated by cell-surface labeling experiments. Saline offers an efficient and non-toxic catalytic option for performing the bioorthogonal-coupling reaction of biomolecules at the physiological pH. This saline mediated bioconjugation reaction represents the most biofriendly, mild and versatile approach for conjugating sensitive biomolecules and does not require any extensive purification step.

U2 - 10.1038/s41598-018-20735-0

DO - 10.1038/s41598-018-20735-0

M3 - Article

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 2193

ER -