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Simultaneous extraction and combined bioluminescent assay of NAD+ and NADH

Tutkimustuotosvertaisarvioitu

Standard

Simultaneous extraction and combined bioluminescent assay of NAD+ and NADH. / Karp, Matti T.; Raunio, Raimo P.; Lövgren, Timo N-E.

julkaisussa: ANALYTICAL BIOCHEMISTRY, Vuosikerta 128, Nro 1, 01.1983, s. 175-180.

Tutkimustuotosvertaisarvioitu

Harvard

Karp, MT, Raunio, RP & Lövgren, TN-E 1983, 'Simultaneous extraction and combined bioluminescent assay of NAD+ and NADH', ANALYTICAL BIOCHEMISTRY, Vuosikerta. 128, Nro 1, Sivut 175-180. https://doi.org/10.1016/0003-2697(83)90359-7

APA

Karp, M. T., Raunio, R. P., & Lövgren, T. N-E. (1983). Simultaneous extraction and combined bioluminescent assay of NAD+ and NADH. ANALYTICAL BIOCHEMISTRY, 128(1), 175-180. https://doi.org/10.1016/0003-2697(83)90359-7

Vancouver

Karp MT, Raunio RP, Lövgren TN-E. Simultaneous extraction and combined bioluminescent assay of NAD+ and NADH. ANALYTICAL BIOCHEMISTRY. 1983 tammi;128(1):175-180. https://doi.org/10.1016/0003-2697(83)90359-7

Author

Karp, Matti T. ; Raunio, Raimo P. ; Lövgren, Timo N-E. / Simultaneous extraction and combined bioluminescent assay of NAD+ and NADH. Julkaisussa: ANALYTICAL BIOCHEMISTRY. 1983 ; Vuosikerta 128, Nro 1. Sivut 175-180.

Bibtex - Lataa

@article{b60db97b6bba49959872bd88e14c66f3,
title = "Simultaneous extraction and combined bioluminescent assay of NAD+ and NADH",
abstract = "A new method for extracting pyridine nucleotides from tissue samples at room temperature that allows the simultaneous extraction of both the oxidized and reduced nucleotide when using a 70{\%} buffered ethanol solution as the extractant has been developed. The extraction efficiencies for NAD+ and NADH were 91 and 102{\%}, respectively. The extraction method was followed by a combined bioluminescent assay of both nucleotides. A bacterial bioluminescent system, which included luciferase and low levels of a NADH-specific oxidoreductase, was used to produce a constant light intensity directly proportional to the amount of NADH in the tissue extract sample. When the NADH had been measured, the NAD+ present in the extract was enzymatically converted to NADH by the addition of alcohol dehydrogenase, after which the second increase in light level was recorded. The sensitivity of the bioluminescent assay presented here is 5 X 10(-14) mol NADH or NAD+ per assay.",
keywords = "Alcohol Oxidoreductases, Animals, Chemical Phenomena, Chemistry, Ethanol, Liver, Luciferases, Luminescent Measurements, Methods, NAD, Oxidation-Reduction, Oxidoreductases, Rats, Vibrio",
author = "Karp, {Matti T.} and Raunio, {Raimo P.} and L{\"o}vgren, {Timo N-E.}",
year = "1983",
month = "1",
doi = "10.1016/0003-2697(83)90359-7",
language = "English",
volume = "128",
pages = "175--180",
journal = "ANALYTICAL BIOCHEMISTRY",
issn = "0003-2697",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",
number = "1",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Simultaneous extraction and combined bioluminescent assay of NAD+ and NADH

AU - Karp, Matti T.

AU - Raunio, Raimo P.

AU - Lövgren, Timo N-E.

PY - 1983/1

Y1 - 1983/1

N2 - A new method for extracting pyridine nucleotides from tissue samples at room temperature that allows the simultaneous extraction of both the oxidized and reduced nucleotide when using a 70% buffered ethanol solution as the extractant has been developed. The extraction efficiencies for NAD+ and NADH were 91 and 102%, respectively. The extraction method was followed by a combined bioluminescent assay of both nucleotides. A bacterial bioluminescent system, which included luciferase and low levels of a NADH-specific oxidoreductase, was used to produce a constant light intensity directly proportional to the amount of NADH in the tissue extract sample. When the NADH had been measured, the NAD+ present in the extract was enzymatically converted to NADH by the addition of alcohol dehydrogenase, after which the second increase in light level was recorded. The sensitivity of the bioluminescent assay presented here is 5 X 10(-14) mol NADH or NAD+ per assay.

AB - A new method for extracting pyridine nucleotides from tissue samples at room temperature that allows the simultaneous extraction of both the oxidized and reduced nucleotide when using a 70% buffered ethanol solution as the extractant has been developed. The extraction efficiencies for NAD+ and NADH were 91 and 102%, respectively. The extraction method was followed by a combined bioluminescent assay of both nucleotides. A bacterial bioluminescent system, which included luciferase and low levels of a NADH-specific oxidoreductase, was used to produce a constant light intensity directly proportional to the amount of NADH in the tissue extract sample. When the NADH had been measured, the NAD+ present in the extract was enzymatically converted to NADH by the addition of alcohol dehydrogenase, after which the second increase in light level was recorded. The sensitivity of the bioluminescent assay presented here is 5 X 10(-14) mol NADH or NAD+ per assay.

KW - Alcohol Oxidoreductases

KW - Animals

KW - Chemical Phenomena

KW - Chemistry

KW - Ethanol

KW - Liver

KW - Luciferases

KW - Luminescent Measurements

KW - Methods

KW - NAD

KW - Oxidation-Reduction

KW - Oxidoreductases

KW - Rats

KW - Vibrio

U2 - 10.1016/0003-2697(83)90359-7

DO - 10.1016/0003-2697(83)90359-7

M3 - Article

VL - 128

SP - 175

EP - 180

JO - ANALYTICAL BIOCHEMISTRY

JF - ANALYTICAL BIOCHEMISTRY

SN - 0003-2697

IS - 1

ER -