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TUTCRIS

Soft tissue compatibility of Li, Sr and B doped bioactive glasses

Tutkimustuotos: Konferenssiesitys, posteri tai abstrakti

Standard

Soft tissue compatibility of Li, Sr and B doped bioactive glasses. / Lyyra, Inari; Sartoneva, Reetta; Kellomäki, Minna; Miettinen, Susanna; Massera, Jonathan.

2017. Julkaisun esittämispaikka: BMT and MED Research Day 2017, Tampere, Suomi.

Tutkimustuotos: Konferenssiesitys, posteri tai abstrakti

Harvard

Lyyra, I, Sartoneva, R, Kellomäki, M, Miettinen, S & Massera, J 2017, 'Soft tissue compatibility of Li, Sr and B doped bioactive glasses' Artikkeli esitetty, Tampere, Suomi, 26/10/17 - 26/10/17, .

APA

Lyyra, I., Sartoneva, R., Kellomäki, M., Miettinen, S., & Massera, J. (2017). Soft tissue compatibility of Li, Sr and B doped bioactive glasses. Julkaisun esittämispaikka: BMT and MED Research Day 2017, Tampere, Suomi.

Vancouver

Lyyra I, Sartoneva R, Kellomäki M, Miettinen S, Massera J. Soft tissue compatibility of Li, Sr and B doped bioactive glasses. 2017. Julkaisun esittämispaikka: BMT and MED Research Day 2017, Tampere, Suomi.

Author

Lyyra, Inari ; Sartoneva, Reetta ; Kellomäki, Minna ; Miettinen, Susanna ; Massera, Jonathan. / Soft tissue compatibility of Li, Sr and B doped bioactive glasses. Julkaisun esittämispaikka: BMT and MED Research Day 2017, Tampere, Suomi.

Bibtex - Lataa

@conference{dae78807f0bd4813a96ea92691e0a5fe,
title = "Soft tissue compatibility of Li, Sr and B doped bioactive glasses",
abstract = "Bioactive glasses are in clinical use in many bone-related applications. The main advantage of these materials comes from the ease of introducing any ions having potential therapeutic effects and controlling their release in the medium. However, little is known about their reactions with soft tissue. In this study, human adipose stem cells (hASC), human lung fibroblasts and urethral epithelium cells were cultured for 14 days in mediums based on 13-93 bioactive glass extracts doped with lithium, strontium or boron. The cell viability, proliferation and phenotype were studied and the ion concentrations in the extract-based mediums were quantified using ICP-OES. The initial results show that the hASC and the fibroblasts remain viable in the extracts and the 13-93 and Li-doped glasses perform as well as the basic medium with hASC. According to the live-dead images, the other glasses slow down the cells proliferation, but these results will be confirmed with the quantitative analysis. Culturing the hASC and fibroblasts in the boron-containing extract seems to increase the cell size. The extracts had a very distinct effect on the morphology of the urethral epithelium cells, especially the Li-containing extract resulted in a very different cell morphology compared to the reference medium. This is most likely due to the high calcium concentration in the extract, which is not well tolerated by epithelial cells.",
keywords = "bioactive glass, soft tissue engineering, ion release, adipose stem cells, fibroblasts, urethral epithelium cells",
author = "Inari Lyyra and Reetta Sartoneva and Minna Kellom{\"a}ki and Susanna Miettinen and Jonathan Massera",
year = "2017",
month = "10",
day = "26",
language = "English",
note = "BMT and MED Research Day 2017 ; Conference date: 26-10-2017 Through 26-10-2017",
url = "http://www.biomeditech.fi/RD2017/",

}

RIS (suitable for import to EndNote) - Lataa

TY - CONF

T1 - Soft tissue compatibility of Li, Sr and B doped bioactive glasses

AU - Lyyra, Inari

AU - Sartoneva, Reetta

AU - Kellomäki, Minna

AU - Miettinen, Susanna

AU - Massera, Jonathan

PY - 2017/10/26

Y1 - 2017/10/26

N2 - Bioactive glasses are in clinical use in many bone-related applications. The main advantage of these materials comes from the ease of introducing any ions having potential therapeutic effects and controlling their release in the medium. However, little is known about their reactions with soft tissue. In this study, human adipose stem cells (hASC), human lung fibroblasts and urethral epithelium cells were cultured for 14 days in mediums based on 13-93 bioactive glass extracts doped with lithium, strontium or boron. The cell viability, proliferation and phenotype were studied and the ion concentrations in the extract-based mediums were quantified using ICP-OES. The initial results show that the hASC and the fibroblasts remain viable in the extracts and the 13-93 and Li-doped glasses perform as well as the basic medium with hASC. According to the live-dead images, the other glasses slow down the cells proliferation, but these results will be confirmed with the quantitative analysis. Culturing the hASC and fibroblasts in the boron-containing extract seems to increase the cell size. The extracts had a very distinct effect on the morphology of the urethral epithelium cells, especially the Li-containing extract resulted in a very different cell morphology compared to the reference medium. This is most likely due to the high calcium concentration in the extract, which is not well tolerated by epithelial cells.

AB - Bioactive glasses are in clinical use in many bone-related applications. The main advantage of these materials comes from the ease of introducing any ions having potential therapeutic effects and controlling their release in the medium. However, little is known about their reactions with soft tissue. In this study, human adipose stem cells (hASC), human lung fibroblasts and urethral epithelium cells were cultured for 14 days in mediums based on 13-93 bioactive glass extracts doped with lithium, strontium or boron. The cell viability, proliferation and phenotype were studied and the ion concentrations in the extract-based mediums were quantified using ICP-OES. The initial results show that the hASC and the fibroblasts remain viable in the extracts and the 13-93 and Li-doped glasses perform as well as the basic medium with hASC. According to the live-dead images, the other glasses slow down the cells proliferation, but these results will be confirmed with the quantitative analysis. Culturing the hASC and fibroblasts in the boron-containing extract seems to increase the cell size. The extracts had a very distinct effect on the morphology of the urethral epithelium cells, especially the Li-containing extract resulted in a very different cell morphology compared to the reference medium. This is most likely due to the high calcium concentration in the extract, which is not well tolerated by epithelial cells.

KW - bioactive glass, soft tissue engineering, ion release, adipose stem cells, fibroblasts, urethral epithelium cells

M3 - Paper, poster or abstract

ER -