TUTCRIS - Tampereen teknillinen yliopisto

TUTCRIS

Superquenching of SYBRGreen dye fluorescence in complex with DNA by gold nanoparticles

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut363-367
Sivumäärä5
JulkaisuHIGH ENERGY CHEMISTRY
Vuosikerta46
Numero6
DOI - pysyväislinkit
TilaJulkaistu - marraskuuta 2012
OKM-julkaisutyyppiA1 Alkuperäisartikkeli

Tiivistelmä

The influence of gold nanoparticles (diameter of about 2.5 nm) on the complex between the SYBRGreen dye and double-stranded DNA in solutions has been investigated by fluorescence spectroscopy. Strong quenching of dye fluorescence by nanosized gold particles ("superquenching"), characterized by a high Stern-Volmer constant of KSV = 3.3 × 107 L/mol, has been found. The superquenching effect in the test system is explained in terms of contribution of several processes: electron transfer, formation of aggregates of gold nanoparticles involving dye dications, and enhancement of intersystem crossing by a heavy atom (gold atoms of nanoparticles).

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