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TUTCRIS

Synchrony between Genetic Repressilators in Sister Cells in Different Temperatures

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
OtsikkoICCMSB 2015: International Conference on Computational and Molecular Systems Biology
KustantajaWorld Academy of Science, Engineering and Technology
Sivut725-728
Sivumäärä4
TilaJulkaistu - 2015
OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisussa
TapahtumaInternational Conference on Computational and Molecular Systems Biology - , Iso-Britannia
Kesto: 1 tammikuuta 2015 → …

Julkaisusarja

NimiProceedings of the World Academy of Science, Engineering and Technology
ISSN (painettu)2010-376X
ISSN (elektroninen)2010-3778

Conference

ConferenceInternational Conference on Computational and Molecular Systems Biology
MaaIso-Britannia
Ajanjakso1/01/15 → …

Tiivistelmä

We used live E. coli containing synthetic genetic oscillators to study how the degree of synchrony between the genetic circuits of sister cells changes with temperature. We found that both the mean and the variability of the degree of synchrony between the fluorescence signals from sister cells are affected by temperature. Also, while most pairs of sister cells were found to be highly synchronous in each condition, the number of asynchronous pairs increased with increasing temperature, which was found to be due to disruptions in the oscillations. Finally we provide evidence that these disruptions tend to affect multiple generations as opposed to individual cells. These findings provide insight in how to design more robust synthetic circuits and in how cell division can affect their dynamics.

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