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Temperature dependence of leakiness of transcription repression mechanisms of Escherichia coli

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
OtsikkoComputational Methods in Systems Biology - 14th International Conference, CMSB 2016
KustantajaSpringer Verlag
Sivut341-342
Sivumäärä2
ISBN (painettu)9783319451763
TilaJulkaistu - 2016
OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisussa
TapahtumaInternational Conference on Computational Methods in Systems Biology -
Kesto: 1 tammikuuta 1900 → …

Julkaisusarja

NimiLecture Notes in Computer Science
Vuosikerta9859
ISSN (painettu)0302-9743
ISSN (elektroninen)1611-3349

Conference

ConferenceInternational Conference on Computational Methods in Systems Biology
Ajanjakso1/01/00 → …

Tiivistelmä

In E. coli, transcription repression is essential in cellular functioning. However, its failure rates are non-negligible. We measured the leakiness rate of lacO3O1 promoter with single RNA sensitivity and its temperature dependence in live cells. After finding strong temperature dependence, we dissected the causes. While RNA polymerase numbers and kt, the rate of active transcription, vary weakly with temperature, the repression strength (dependent on number of repressors and binding and unbinding rates of repressors to the promoter) is heavily temperature dependent. We conclude that the lacO3O1 leakiness at low temperatures increases as the repression mechanism’s efficiency hampers.

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