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The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells

Tutkimustuotosvertaisarvioitu

Standard

The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells. / Virjula, Sanni; Zhao, Feihu; Leivo, Joni; Vanhatupa, Sari; Kreutzer, Joose; Vaughan, Ted J.; Honkala, Anna Maija; Viehrig, Marlitt; Mullen, Conleth A.; Kallio, Pasi; McNamara, Laoise M.; Miettinen, Susanna.

julkaisussa: Journal of the Mechanical Behavior of Biomedical Materials, Vuosikerta 72, 01.08.2017, s. 38-48.

Tutkimustuotosvertaisarvioitu

Harvard

Virjula, S, Zhao, F, Leivo, J, Vanhatupa, S, Kreutzer, J, Vaughan, TJ, Honkala, AM, Viehrig, M, Mullen, CA, Kallio, P, McNamara, LM & Miettinen, S 2017, 'The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells', Journal of the Mechanical Behavior of Biomedical Materials, Vuosikerta. 72, Sivut 38-48. https://doi.org/10.1016/j.jmbbm.2017.04.016

APA

Virjula, S., Zhao, F., Leivo, J., Vanhatupa, S., Kreutzer, J., Vaughan, T. J., ... Miettinen, S. (2017). The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells. Journal of the Mechanical Behavior of Biomedical Materials, 72, 38-48. https://doi.org/10.1016/j.jmbbm.2017.04.016

Vancouver

Virjula S, Zhao F, Leivo J, Vanhatupa S, Kreutzer J, Vaughan TJ et al. The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells. Journal of the Mechanical Behavior of Biomedical Materials. 2017 elo 1;72:38-48. https://doi.org/10.1016/j.jmbbm.2017.04.016

Author

Virjula, Sanni ; Zhao, Feihu ; Leivo, Joni ; Vanhatupa, Sari ; Kreutzer, Joose ; Vaughan, Ted J. ; Honkala, Anna Maija ; Viehrig, Marlitt ; Mullen, Conleth A. ; Kallio, Pasi ; McNamara, Laoise M. ; Miettinen, Susanna. / The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells. Julkaisussa: Journal of the Mechanical Behavior of Biomedical Materials. 2017 ; Vuosikerta 72. Sivut 38-48.

Bibtex - Lataa

@article{cf7d2d72cedb4857b713490d53973347,
title = "The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells",
abstract = "Although mechanical cues are known to affect stem cell fate and mechanobiology, the significance of such stimuli on the osteogenic differentiation of human adipose stem cells (hASCs) remains unclear. In this study, we investigated the effect of long-term mechanical stimulation on the attachment, osteogenic differentiation and mechanical properties of hASCs. Tailor-made, pneumatic cell stretching devices were used to expose hASCs to cyclic equiaxial stretching in osteogenic medium. Cell attachment and focal adhesions were visualised using immunocytochemical vinculin staining on days 3 and 6, and the proliferation and alkaline phosphatase activity, as a sign of early osteogenic differentiation, were analysed on days 0, 6 and 10. Furthermore, the mechanical properties of hASCs, in terms of apparent Young's modulus and normalised contractility, were obtained using a combination of atomic force microscopy based indentation and computational approaches. Our results indicated that cyclic equiaxial stretching delayed proliferation and promoted osteogenic differentiation of hASCs. Stretching also reduced cell size and intensified focal adhesions and actin cytoskeleton. Moreover, cell stiffening was observed during osteogenic differentiation and especially under mechanical stimulation. These results suggest that cyclic equiaxial stretching modifies cell morphology, focal adhesion formation and mechanical properties of hASCs. This could be exploited to enhance osteogenic differentiation.",
keywords = "Atomic force microscopy indentation, Dynamic cell culture, Mechanical stimulation, mechanobiology, Mesenchymal stem cell, PDMS",
author = "Sanni Virjula and Feihu Zhao and Joni Leivo and Sari Vanhatupa and Joose Kreutzer and Vaughan, {Ted J.} and Honkala, {Anna Maija} and Marlitt Viehrig and Mullen, {Conleth A.} and Pasi Kallio and McNamara, {Laoise M.} and Susanna Miettinen",
note = "EXT={"}Zhao, Feihu{"} INT=tut-bmt,”Viehrig, Marlitt”",
year = "2017",
month = "8",
day = "1",
doi = "10.1016/j.jmbbm.2017.04.016",
language = "English",
volume = "72",
pages = "38--48",
journal = "Journal of the Mechanical Behavior of Biomedical Materials",
issn = "1751-6161",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells

AU - Virjula, Sanni

AU - Zhao, Feihu

AU - Leivo, Joni

AU - Vanhatupa, Sari

AU - Kreutzer, Joose

AU - Vaughan, Ted J.

AU - Honkala, Anna Maija

AU - Viehrig, Marlitt

AU - Mullen, Conleth A.

AU - Kallio, Pasi

AU - McNamara, Laoise M.

AU - Miettinen, Susanna

N1 - EXT="Zhao, Feihu" INT=tut-bmt,”Viehrig, Marlitt”

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Although mechanical cues are known to affect stem cell fate and mechanobiology, the significance of such stimuli on the osteogenic differentiation of human adipose stem cells (hASCs) remains unclear. In this study, we investigated the effect of long-term mechanical stimulation on the attachment, osteogenic differentiation and mechanical properties of hASCs. Tailor-made, pneumatic cell stretching devices were used to expose hASCs to cyclic equiaxial stretching in osteogenic medium. Cell attachment and focal adhesions were visualised using immunocytochemical vinculin staining on days 3 and 6, and the proliferation and alkaline phosphatase activity, as a sign of early osteogenic differentiation, were analysed on days 0, 6 and 10. Furthermore, the mechanical properties of hASCs, in terms of apparent Young's modulus and normalised contractility, were obtained using a combination of atomic force microscopy based indentation and computational approaches. Our results indicated that cyclic equiaxial stretching delayed proliferation and promoted osteogenic differentiation of hASCs. Stretching also reduced cell size and intensified focal adhesions and actin cytoskeleton. Moreover, cell stiffening was observed during osteogenic differentiation and especially under mechanical stimulation. These results suggest that cyclic equiaxial stretching modifies cell morphology, focal adhesion formation and mechanical properties of hASCs. This could be exploited to enhance osteogenic differentiation.

AB - Although mechanical cues are known to affect stem cell fate and mechanobiology, the significance of such stimuli on the osteogenic differentiation of human adipose stem cells (hASCs) remains unclear. In this study, we investigated the effect of long-term mechanical stimulation on the attachment, osteogenic differentiation and mechanical properties of hASCs. Tailor-made, pneumatic cell stretching devices were used to expose hASCs to cyclic equiaxial stretching in osteogenic medium. Cell attachment and focal adhesions were visualised using immunocytochemical vinculin staining on days 3 and 6, and the proliferation and alkaline phosphatase activity, as a sign of early osteogenic differentiation, were analysed on days 0, 6 and 10. Furthermore, the mechanical properties of hASCs, in terms of apparent Young's modulus and normalised contractility, were obtained using a combination of atomic force microscopy based indentation and computational approaches. Our results indicated that cyclic equiaxial stretching delayed proliferation and promoted osteogenic differentiation of hASCs. Stretching also reduced cell size and intensified focal adhesions and actin cytoskeleton. Moreover, cell stiffening was observed during osteogenic differentiation and especially under mechanical stimulation. These results suggest that cyclic equiaxial stretching modifies cell morphology, focal adhesion formation and mechanical properties of hASCs. This could be exploited to enhance osteogenic differentiation.

KW - Atomic force microscopy indentation

KW - Dynamic cell culture

KW - Mechanical stimulation

KW - mechanobiology

KW - Mesenchymal stem cell

KW - PDMS

U2 - 10.1016/j.jmbbm.2017.04.016

DO - 10.1016/j.jmbbm.2017.04.016

M3 - Article

VL - 72

SP - 38

EP - 48

JO - Journal of the Mechanical Behavior of Biomedical Materials

JF - Journal of the Mechanical Behavior of Biomedical Materials

SN - 1751-6161

ER -