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TUTCRIS

The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers

Tutkimustuotosvertaisarvioitu

Standard

The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers. / Lolicato, Fabio; Joly, Loic; Martinez-Seara, Hector; Fragneto, Giovanna; Scoppola, Ernesto; Baldelli Bombelli, Francesca; Vattulainen, Ilpo; Akola, Jaakko; Maccarini, Marco.

julkaisussa: Small, Vuosikerta 15, Nro 23, 1805046, 07.06.2019.

Tutkimustuotosvertaisarvioitu

Harvard

Lolicato, F, Joly, L, Martinez-Seara, H, Fragneto, G, Scoppola, E, Baldelli Bombelli, F, Vattulainen, I, Akola, J & Maccarini, M 2019, 'The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers', Small, Vuosikerta. 15, Nro 23, 1805046. https://doi.org/10.1002/smll.201805046

APA

Lolicato, F., Joly, L., Martinez-Seara, H., Fragneto, G., Scoppola, E., Baldelli Bombelli, F., ... Maccarini, M. (2019). The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers. Small, 15(23), [1805046]. https://doi.org/10.1002/smll.201805046

Vancouver

Lolicato F, Joly L, Martinez-Seara H, Fragneto G, Scoppola E, Baldelli Bombelli F et al. The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers. Small. 2019 kesä 7;15(23). 1805046. https://doi.org/10.1002/smll.201805046

Author

Lolicato, Fabio ; Joly, Loic ; Martinez-Seara, Hector ; Fragneto, Giovanna ; Scoppola, Ernesto ; Baldelli Bombelli, Francesca ; Vattulainen, Ilpo ; Akola, Jaakko ; Maccarini, Marco. / The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers. Julkaisussa: Small. 2019 ; Vuosikerta 15, Nro 23.

Bibtex - Lataa

@article{6cc662ae46684d96bc66880926346822,
title = "The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers",
abstract = "Understanding the molecular mechanisms governing nanoparticle–membrane interactions is of prime importance for drug delivery and biomedical applications. Neutron reflectometry (NR) experiments are combined with atomistic and coarse-grained molecular dynamics (MD) simulations to study the interaction between cationic gold nanoparticles (AuNPs) and model lipid membranes composed of a mixture of zwitterionic di-stearoyl-phosphatidylcholine (DSPC) and anionic di-stearoyl-phosphatidylglycerol (DSPG). MD simulations show that the interaction between AuNPs and a pure DSPC lipid bilayer is modulated by a free energy barrier. This can be overcome by increasing temperature, which promotes an irreversible AuNP incorporation into the lipid bilayer. NR experiments confirm the encapsulation of the AuNPs within the lipid bilayer at temperatures around 55 °C. In contrast, the AuNP adsorption is weak and impaired by heating for a DSPC–DSPG (3:1) lipid bilayer. These results demonstrate that both the lipid charge and the temperature play pivotal roles in AuNP–membrane interactions. Furthermore, NR experiments indicate that the (negative) DSPG lipids are associated with lipid extraction upon AuNP adsorption, which is confirmed by coarse-grained MD simulations as a lipid-crawling effect driving further AuNP aggregation. Overall, the obtained detailed molecular view of the interaction mechanisms sheds light on AuNP incorporation and membrane destabilization.",
keywords = "gold nanoparticles, lipid membranes, molecular dynamics simulations, nanotoxicity, neutron reflectometry",
author = "Fabio Lolicato and Loic Joly and Hector Martinez-Seara and Giovanna Fragneto and Ernesto Scoppola and {Baldelli Bombelli}, Francesca and Ilpo Vattulainen and Jaakko Akola and Marco Maccarini",
note = "EXT={"}Martinez-Seara, Hector{"}",
year = "2019",
month = "6",
day = "7",
doi = "10.1002/smll.201805046",
language = "English",
volume = "15",
journal = "Small",
issn = "1613-6810",
publisher = "Wiley-VCH Verlag",
number = "23",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers

AU - Lolicato, Fabio

AU - Joly, Loic

AU - Martinez-Seara, Hector

AU - Fragneto, Giovanna

AU - Scoppola, Ernesto

AU - Baldelli Bombelli, Francesca

AU - Vattulainen, Ilpo

AU - Akola, Jaakko

AU - Maccarini, Marco

N1 - EXT="Martinez-Seara, Hector"

PY - 2019/6/7

Y1 - 2019/6/7

N2 - Understanding the molecular mechanisms governing nanoparticle–membrane interactions is of prime importance for drug delivery and biomedical applications. Neutron reflectometry (NR) experiments are combined with atomistic and coarse-grained molecular dynamics (MD) simulations to study the interaction between cationic gold nanoparticles (AuNPs) and model lipid membranes composed of a mixture of zwitterionic di-stearoyl-phosphatidylcholine (DSPC) and anionic di-stearoyl-phosphatidylglycerol (DSPG). MD simulations show that the interaction between AuNPs and a pure DSPC lipid bilayer is modulated by a free energy barrier. This can be overcome by increasing temperature, which promotes an irreversible AuNP incorporation into the lipid bilayer. NR experiments confirm the encapsulation of the AuNPs within the lipid bilayer at temperatures around 55 °C. In contrast, the AuNP adsorption is weak and impaired by heating for a DSPC–DSPG (3:1) lipid bilayer. These results demonstrate that both the lipid charge and the temperature play pivotal roles in AuNP–membrane interactions. Furthermore, NR experiments indicate that the (negative) DSPG lipids are associated with lipid extraction upon AuNP adsorption, which is confirmed by coarse-grained MD simulations as a lipid-crawling effect driving further AuNP aggregation. Overall, the obtained detailed molecular view of the interaction mechanisms sheds light on AuNP incorporation and membrane destabilization.

AB - Understanding the molecular mechanisms governing nanoparticle–membrane interactions is of prime importance for drug delivery and biomedical applications. Neutron reflectometry (NR) experiments are combined with atomistic and coarse-grained molecular dynamics (MD) simulations to study the interaction between cationic gold nanoparticles (AuNPs) and model lipid membranes composed of a mixture of zwitterionic di-stearoyl-phosphatidylcholine (DSPC) and anionic di-stearoyl-phosphatidylglycerol (DSPG). MD simulations show that the interaction between AuNPs and a pure DSPC lipid bilayer is modulated by a free energy barrier. This can be overcome by increasing temperature, which promotes an irreversible AuNP incorporation into the lipid bilayer. NR experiments confirm the encapsulation of the AuNPs within the lipid bilayer at temperatures around 55 °C. In contrast, the AuNP adsorption is weak and impaired by heating for a DSPC–DSPG (3:1) lipid bilayer. These results demonstrate that both the lipid charge and the temperature play pivotal roles in AuNP–membrane interactions. Furthermore, NR experiments indicate that the (negative) DSPG lipids are associated with lipid extraction upon AuNP adsorption, which is confirmed by coarse-grained MD simulations as a lipid-crawling effect driving further AuNP aggregation. Overall, the obtained detailed molecular view of the interaction mechanisms sheds light on AuNP incorporation and membrane destabilization.

KW - gold nanoparticles

KW - lipid membranes

KW - molecular dynamics simulations

KW - nanotoxicity

KW - neutron reflectometry

U2 - 10.1002/smll.201805046

DO - 10.1002/smll.201805046

M3 - Article

VL - 15

JO - Small

JF - Small

SN - 1613-6810

IS - 23

M1 - 1805046

ER -