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Time-Resolved Fluorescence Spectroscopy Reveals Fine Structure and Dynamics of Poly(l-lysine) and Polyethylenimine Based DNA Polyplexes

Tutkimustuotosvertaisarvioitu

Standard

Time-Resolved Fluorescence Spectroscopy Reveals Fine Structure and Dynamics of Poly(l-lysine) and Polyethylenimine Based DNA Polyplexes. / Lisitsyna, Ekaterina S.; Ketola, Tiia-Maaria; Morin-Picardat, Emmanuelle; Liang, Huamin; Hanzlíková, Martina; Urtti, Arto; Yliperttula, Marjo; Vuorimaa-Laukkanen, Elina.

julkaisussa: Journal of Physical Chemistry B, Vuosikerta 121, Nro 48, 07.12.2017, s. 10782-10792.

Tutkimustuotosvertaisarvioitu

Harvard

Lisitsyna, ES, Ketola, T-M, Morin-Picardat, E, Liang, H, Hanzlíková, M, Urtti, A, Yliperttula, M & Vuorimaa-Laukkanen, E 2017, 'Time-Resolved Fluorescence Spectroscopy Reveals Fine Structure and Dynamics of Poly(l-lysine) and Polyethylenimine Based DNA Polyplexes', Journal of Physical Chemistry B, Vuosikerta. 121, Nro 48, Sivut 10782-10792. https://doi.org/10.1021/acs.jpcb.7b08394

APA

Vancouver

Lisitsyna ES, Ketola T-M, Morin-Picardat E, Liang H, Hanzlíková M, Urtti A et al. Time-Resolved Fluorescence Spectroscopy Reveals Fine Structure and Dynamics of Poly(l-lysine) and Polyethylenimine Based DNA Polyplexes. Journal of Physical Chemistry B. 2017 joulu 7;121(48):10782-10792. https://doi.org/10.1021/acs.jpcb.7b08394

Author

Lisitsyna, Ekaterina S. ; Ketola, Tiia-Maaria ; Morin-Picardat, Emmanuelle ; Liang, Huamin ; Hanzlíková, Martina ; Urtti, Arto ; Yliperttula, Marjo ; Vuorimaa-Laukkanen, Elina. / Time-Resolved Fluorescence Spectroscopy Reveals Fine Structure and Dynamics of Poly(l-lysine) and Polyethylenimine Based DNA Polyplexes. Julkaisussa: Journal of Physical Chemistry B. 2017 ; Vuosikerta 121, Nro 48. Sivut 10782-10792.

Bibtex - Lataa

@article{ad4074ab875847258d6e8081e2a2cefc,
title = "Time-Resolved Fluorescence Spectroscopy Reveals Fine Structure and Dynamics of Poly(l-lysine) and Polyethylenimine Based DNA Polyplexes",
abstract = "Structural dynamics of the polyethylenimine-DNA and poly(l-lysine)-DNA complexes (polyplexes) was studied by steady-state and time-resolved fluorescence spectroscopy using the fluorescence resonance energy transfer (FRET) technique. During the formation of the DNA polyplexes, the negative phosphate groups (P) of DNA are bound by the positive amine groups (N) of the polymer. At N/P ratio 2, nearly all of the DNA's P groups are bound by the polymer N groups: These complexes form the core of the polyplexes. The excess polymer, added to this system to increase the N/P ratio to the values giving efficient gene delivery, forms a positively charged shell around the core polyplex. We investigated whether the exchange between the core and shell regions of PEI and PLL polyplexes takes place. Our results demonstrated a clear difference between the two studied polymers. Shell PEI can replace PEIs previously attached to DNA in the polyplex core, while PLL cannot. Such a dynamic structure of PEI polyplexes compared to a more static one found for PLL polyplexes partially explains the observed difference in the DNA transfection efficiency of these polyplexes. Moreover, the time-resolved fluorescence spectroscopy revealed additional details on the structure of PLL polyplexes: In between the core and shell, there is an intermediate layer where both core and shell PLLs or their parts overlap.",
author = "Lisitsyna, {Ekaterina S.} and Tiia-Maaria Ketola and Emmanuelle Morin-Picardat and Huamin Liang and Martina Hanzl{\'i}kov{\'a} and Arto Urtti and Marjo Yliperttula and Elina Vuorimaa-Laukkanen",
year = "2017",
month = "12",
day = "7",
doi = "10.1021/acs.jpcb.7b08394",
language = "English",
volume = "121",
pages = "10782--10792",
journal = "Journal of Physical Chemistry Part B",
issn = "1520-6106",
publisher = "American Chemical Society",
number = "48",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Time-Resolved Fluorescence Spectroscopy Reveals Fine Structure and Dynamics of Poly(l-lysine) and Polyethylenimine Based DNA Polyplexes

AU - Lisitsyna, Ekaterina S.

AU - Ketola, Tiia-Maaria

AU - Morin-Picardat, Emmanuelle

AU - Liang, Huamin

AU - Hanzlíková, Martina

AU - Urtti, Arto

AU - Yliperttula, Marjo

AU - Vuorimaa-Laukkanen, Elina

PY - 2017/12/7

Y1 - 2017/12/7

N2 - Structural dynamics of the polyethylenimine-DNA and poly(l-lysine)-DNA complexes (polyplexes) was studied by steady-state and time-resolved fluorescence spectroscopy using the fluorescence resonance energy transfer (FRET) technique. During the formation of the DNA polyplexes, the negative phosphate groups (P) of DNA are bound by the positive amine groups (N) of the polymer. At N/P ratio 2, nearly all of the DNA's P groups are bound by the polymer N groups: These complexes form the core of the polyplexes. The excess polymer, added to this system to increase the N/P ratio to the values giving efficient gene delivery, forms a positively charged shell around the core polyplex. We investigated whether the exchange between the core and shell regions of PEI and PLL polyplexes takes place. Our results demonstrated a clear difference between the two studied polymers. Shell PEI can replace PEIs previously attached to DNA in the polyplex core, while PLL cannot. Such a dynamic structure of PEI polyplexes compared to a more static one found for PLL polyplexes partially explains the observed difference in the DNA transfection efficiency of these polyplexes. Moreover, the time-resolved fluorescence spectroscopy revealed additional details on the structure of PLL polyplexes: In between the core and shell, there is an intermediate layer where both core and shell PLLs or their parts overlap.

AB - Structural dynamics of the polyethylenimine-DNA and poly(l-lysine)-DNA complexes (polyplexes) was studied by steady-state and time-resolved fluorescence spectroscopy using the fluorescence resonance energy transfer (FRET) technique. During the formation of the DNA polyplexes, the negative phosphate groups (P) of DNA are bound by the positive amine groups (N) of the polymer. At N/P ratio 2, nearly all of the DNA's P groups are bound by the polymer N groups: These complexes form the core of the polyplexes. The excess polymer, added to this system to increase the N/P ratio to the values giving efficient gene delivery, forms a positively charged shell around the core polyplex. We investigated whether the exchange between the core and shell regions of PEI and PLL polyplexes takes place. Our results demonstrated a clear difference between the two studied polymers. Shell PEI can replace PEIs previously attached to DNA in the polyplex core, while PLL cannot. Such a dynamic structure of PEI polyplexes compared to a more static one found for PLL polyplexes partially explains the observed difference in the DNA transfection efficiency of these polyplexes. Moreover, the time-resolved fluorescence spectroscopy revealed additional details on the structure of PLL polyplexes: In between the core and shell, there is an intermediate layer where both core and shell PLLs or their parts overlap.

U2 - 10.1021/acs.jpcb.7b08394

DO - 10.1021/acs.jpcb.7b08394

M3 - Article

VL - 121

SP - 10782

EP - 10792

JO - Journal of Physical Chemistry Part B

JF - Journal of Physical Chemistry Part B

SN - 1520-6106

IS - 48

ER -