TUTCRIS - Tampereen teknillinen yliopisto

TUTCRIS

Transcription closed and open complex formation coordinate expression of genes with a shared promoter region

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Yksityiskohdat

AlkuperäiskieliEnglanti
Artikkeli20190507
Sivumäärä11
JulkaisuJournal of the Royal Society Interface
Vuosikerta16
Numero161
DOI - pysyväislinkit
TilaJulkaistu - 1 joulukuuta 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli

Tiivistelmä

Many genes are spaced closely, allowing coordination without explicit control through shared regulatory elements and molecular interactions. We study the dynamics of a stochastic model of a gene-pair in a head-to-head configuration, sharing promoter elements, which accounts for the rate-limiting steps in transcription initiation. We find that only in specific regions of the parameter space of the rate-limiting steps is orderly coexpression exhibited, suggesting that successful cooperation between closely spaced genes requires the coevolution of compatible rate-limiting step configuration. The model predictions are validated using in vivo single-cell, single-RNA measurements of the dynamics of pairs of genes sharing promoter elements. Our results suggest that, in E. coli, the kinetics of the rate-limiting steps in active transcription can play a central role in shaping the dynamics of gene-pairs sharing promoter elements.

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