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TUTCRIS

X-ray microtomography of collagen and polylactide samples in liquids

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
OtsikkoXIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016
AlaotsikkoMEDICON 2016, March 31st–April 2nd 2016, Paphos, Cyprus
KustantajaSpringer Verlag
Sivut420-424
Sivumäärä5
ISBN (painettu)9783319327013
DOI - pysyväislinkit
TilaJulkaistu - 2016
OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisussa
TapahtumaMediterranean Conference on Medical and Biological Engineering and Computing -
Kesto: 1 tammikuuta 2000 → …

Julkaisusarja

NimiIFMBE Proceedings
Vuosikerta57
ISSN (painettu)1680-0737

Conference

ConferenceMediterranean Conference on Medical and Biological Engineering and Computing
Ajanjakso1/01/00 → …

Tiivistelmä

Methods to image and assess the microstructure of polymer based biomaterials in liquid phase, for example cell culture medium, are well warranted. X-ray microtomography could provide a mean to visualize and analyze such structures. However, the density of such polymers is close to that of water and hence the inherent X-ray contrast is poor. The material can provide good contrast when dry, however, if the materials contain cells and are dried, the cell morphology may be distorted. Moreover the entire structure of these water containing materials are deformed in the drying process. In this paper we tested phosphotungstic acid (PTA) staining to improve the contrast. We imaged collagen and PLA samples, as well as collagen-PLA composites with μCT in air, water and alcohol. The methods were compared visually and with contrast to noise ratio calculated from the images. Our results demonstrate that with alcohol the PLA can be imaged also in liquid phase. PTA staining seems to be a good method to increase the contrast for collagen in μCT imaging.

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